Resource announcement: Genome sequences for the wheat stripe rust pathogen (Puccinia striiformis f. sp. tritici) and the barley stripe rust pathogen (Puccinia striiformis f. sp. hordei)

Authors: XIA, CHONGJING - Washington State University; WANG, MEINAN - Washington State University; YIN, CHUNTAO - Washington State University; CORNEJO, OMAR - Washington State University; HULBERT, SCOT - Washington State University; Chen, Xianming

Submitted to: Molecular Plant-Microbe Interactions
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 5/23/2018
Publication Date: 5/24/2018
Citation: Xia, C., Wang, M., Yin, C., Cornejo, O.E., Hulbert, S., Chen, X. 2018. Resource announcement: Genome sequences for the wheat stripe rust pathogen (Puccinia striiformis f. sp. tritici) and the barley stripe rust pathogen (Puccinia striiformis f. sp. hordei). Molecular Plant-Microbe Interactions. https://doi.org/10.1094/MPMI-04-18-0107-A.
DOI: https://doi.org/10.1094/MPMI-04-18-0107-A

Interpretive Summary: Puccinia striiformis f. sp. tritici (Pst) causes devastating stripe rust on wheat and P. striiformis f. sp. hordei (Psh) causes stripe rust on barley. Several genomes of Pst have been sequenced, but none of Psh genomes are available. More genome sequences from both Pst and Psh are needed to understand the genome evolution and identify molecular mechanisms of their pathogenicitys. We sequenced Pst isolate 93-210 and Psh isolate 93TX-2 using the combination of PacBio and Illumina technologies and RNA sequencing. The circular mitochondria genomes for both Pst and Psh were complete and almost identical. Their genomic sequences were assembled to contigs with high continuity and showed significant structural differences. These genomes provide high-quality resources for deciphering the genomic basis of rapid evolution and host adaptation, identifying genes for avirulence and other important traits, and studying the pathogen-host interactions.

Technical Abstract: Puccinia striiformis f. sp. tritici (Pst) causes devastating stripe (yellow) rust on wheat and P. striiformis f. sp. hordei (Psh) causes stripe rust on barley. Several genomes of Pst have been sequenced, but none of Psh genomes are available. More genome sequences from both Pst and Psh are needed to understand the genome evolution and identify molecular mechanisms of their pathogenicitys. We sequenced Pst isolate 93-210 and Psh isolate 93TX-2 using the combination of PacBio and Illumina technologies and RNA sequencing. The circular mitochondria genomes for both Pst and Psh were complete and almost identical. Their genomic sequences were assembled to contigs with high continuity and showed significant structural differences. These genomes provide high-quality resources for deciphering the genomic basis of rapid evolution and host adaptation, identifying genes for avirulence and other important traits, and studying the pathogen-host interactions.