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ARS Home » Pacific West Area » Pullman, Washington » Plant Germplasm Introduction and Testing Research » Research » Publications at this Location » Publication #352489

Research Project: Management of Priority Legume, Oilseed, Vegetable, Forage Grass, Sugar, Ornamental, and Medicinal Plant Genetic Resources and Associated Information

Location: Plant Germplasm Introduction and Testing Research

Title: Enhancing faba bean (Vicia faba L.) germplasm for resilience to temperature extremes

Author
item Hu, Jinguo
item Maalouf, Fouad - International Center For Agricultural Research In The Dry Areas (ICARDA)
item Dong, Haixiao - Washington State University
item Hawkins, Charles - Washington State University
item Zhang, Zhiwu - Washington State University
item Ma, Yu - Washington State University
item Coyne, Clarice - Clare
item Jin, Deming - Huazhong Agricultural University
item Yu, Long Xi
item Babiker, Zayed - Agricultural Research Corporation
item Hamwieh, Aladdin - International Center For Agricultural Research In The Dry Areas (ICARDA)
item Khater, Lynn Abou - International Center For Agricultural Research In The Dry Areas (ICARDA)
item Baum, Michael - International Center For Agricultural Research In The Dry Areas (ICARDA)

Submitted to: Meeting Abstract
Publication Type: Abstract Only
Publication Acceptance Date: 3/13/2018
Publication Date: N/A
Citation: N/A

Interpretive Summary: none

Technical Abstract: The Western Regional Plant Introduction Station (WRPIS) is one of the four regional plant introduction stations in the US Department of Agriculture-ARS National Plant Germplasm System (NPGS) with the mission of acquiring, documenting, maintaining, characterizing, evaluating and distributing plant genetic resources to the global research community to improve agricultural productivity for food security. Our research on enhancement of faba bean (Vicia faba L.) has the objectives of increasing resilience to temperature extremes and improving nutritional quality. We conduct traditional field/greenhouse experiments and adopt contemporary genomics approaches in our independent and collaborative research projects. In collaboration with the International Center for Agricultural Research in the Dry Areas, Terbol, Lebanon and supported by a grant from the CGIAR-U.S. University Linkages Program, we collected data for eight agronomic traits on a Generation Challenge Program (GCP) reference set of 140 faba bean accessions grown under high temperature at different locations in Lebanon and USA. We also genotyped the reference set with the genotyping by sequencing (GBS) technique which produced approximately 4 million reads per genotype on an Illumina HiSeq 2500 Sequencer. The single nucleotide polymorphism (SNP) marker calling pipeline UNEAK (Universal Network Enabled Analysis Kit) found 10,950 variant loci from the sequence data. After fileting SNPs with missing rate over 60% and minor allele frequency less than 5%, 2,632 SNPs were obtained for final analyses. The final dataset contained 131 accessions with SNP call rate above 70%. The missing SNPs were imputed by LinkImpute. Genome-Wide Association Study (GWAS) was performed using the program BLINK (Bayesian-information and Linkage-disequilibrium Iteratively Nested Keyway). Four SNP markers were identified to be significantly associated with plant height (2 SNPs) and seed size (2SNPs).Currently, the reference set is being evaluated along with some advanced breeding materials for winter survival at Pullman Washington, USA.