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ARS Home » Pacific West Area » Hilo, Hawaii » Daniel K. Inouye U.S. Pacific Basin Agricultural Research Center » Tropical Crop and Commodity Protection Research » Research » Publications at this Location » Publication #352306

Research Project: Detection, Control and Area-wide Management of Fruit Flies and Other Quarantine Pests of Tropical/Subtropical Crops

Location: Tropical Crop and Commodity Protection Research

Title: Mitochondrial genomes of Anopheles arabiensis, An.gambiae and An.coluzzii show no clear species division

Author
item Hanemaaijer, Mark - University Of California, Davis
item Houston, Parker - University Of California, Davis
item Collier, Travis
item Norris, Laura - Gates Foundation
item Fofana, Abdrahamane - University Of Bamako
item Lanzaro, Gregory - University Of California, Davis
item Cornel, Anthony - Kearney Agricultural Center

Submitted to: F1000Research
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 3/21/2018
Publication Date: 3/21/2018
Citation: Hanemaaijer, M.J., Houston, P.D., Collier, T.C., Norris, L.C., Fofana, A., Lanzaro, G.C., Cornel, A.J. 2018. Mitochondrial genomes of Anopheles arabiensis, An.gambiae and An.coluzzii show no clear species division. F1000Research. https://doi.org/10.12688/f1000research.13807.1.
DOI: https://doi.org/10.12688/f1000research.13807.1

Interpretive Summary: Based on 70 samples, the mitochondrial sequences of the three main malaria transmitting mosquito species in Africa show no clear divisions. This is consistent with, but not proof of, hybridization and introgression among these taxa.

Technical Abstract: Here we report the complete mitochondrial sequences of 70 individual field collected mosquito specimens from throughout Sub-Saharan Africa. We generated this dataset to identify species specific markers for the following Anopheles species and chromosomal forms: An.arabiensis, An.coluzzii (The Forest and Mopti chromosomal forms) and An.gambiae (The Bamako and Savannah chromosomal forms). The raw Illumina sequencing reads were mapped to the NC_002084 reference mitogenome sequence. A total of 783 single nucleotide polymorphisms (SNPs) were detected on the mitochondrial genome, of which 460 are singletons (58.7%). None of these SNPs are suitable as molecular markers to distinguish among An.arabiensis, An.coluzzii and An.gambiae or any of the chromosomal forms. The lack of species or chromosomal form specific markers is also reflected in the constructed phylogenetic tree, which shows no clear division among the operational taxonomic units considered here.