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ARS Home » Pacific West Area » Pullman, Washington » Plant Germplasm Introduction and Testing Research » Research » Publications at this Location » Publication #352255

Research Project: Management of Priority Legume, Oilseed, Vegetable, Forage Grass, Sugar, Ornamental, and Medicinal Plant Genetic Resources and Associated Information

Location: Plant Germplasm Introduction and Testing Research

Title: Characterizing Chickpeas for End Use Characteristics

Author
item Coyne, Clarice - Clare
item Zheng, Ping - Washington State University
item Ganjyal, Girish - Washington State University
item Vandemark, George
item Mcgee, Rebecca
item Main, Doreen - Washington State University

Submitted to: Meeting Abstract
Publication Type: Abstract Only
Publication Acceptance Date: 2/15/2018
Publication Date: N/A
Citation: N/A

Interpretive Summary: none

Technical Abstract: In the United States, cool-season grain legumes, including chickpeas, are integral components of cereal-based cropping systems in the Pacific Northwest and the Upper Midwest. The addition of a pulse crop helps break disease and weed cycles and adds nitrogen and organic matter to the soil. In this project, we are working to characterize the USDA Chickpea Core Collection as whole seed and as flour. As a start, using GBS, we genotyped a USDA kabuli mini-core collection of 88 accessions, discovering 174,040 variants. Using the software TASSEL to identify polymorphisms, 47,733 SNPs were identified using no-missing data criteria. The SNP numbers per chromosome were (1) 6296, (2) 4483, (3) 4330, (4) 9258, (5) 4471, (6) 6407, (7) 5917 and (8) 1571. Further genotyping is underway on the remaining 300 lines from the USDA chickpea core collection. We plan to use this resource for GWAS of end-use characteristics. Seed of the kabuli mini-core is available from USDA GRIN Global (https://npgsweb.ars-grin.gov/gringlobal/search.aspx) and the SNP data is available on the CSFL database (https://www.coolseasonfoodlegume.org/).