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ARS Home » Northeast Area » Wyndmoor, Pennsylvania » Eastern Regional Research Center » Food Safety and Intervention Technologies Research » Research » Publications at this Location » Publication #352164

Research Project: The Role of Genotype in the Development and Validation of Growth Models and Intervention Technologies for Pathogenic Non-Shiga Toxigenic Escherichia coli Found in Foods

Location: Food Safety and Intervention Technologies Research

Title: Draft genomic sequencing of six potential extraintestinal pathogenic Escherichia coli isolates from retail chicken meat

Author
item Xu, Aixia - Oak Ridge Institute For Science And Education (ORISE)
item Johnson, James - Department Of Veterans Affairs
item Sheen, Shiowshuh
item Needleman, David
item Sommers, Christopher

Submitted to: Genome Announcements
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 4/18/2018
Publication Date: 5/24/2018
Citation: Xu, A., Johnson, J., Sheen, S., Needleman, D.S., Sommers, C.H. 2018. Draft genomic sequencing of six potential extraintestinal pathogenic Escherichia coli isolates from retail chicken meat. Genome Announcements. https://doi.org/10.1128/genomeA.00449-18.
DOI: https://doi.org/10.1128/genomeA.00449-18

Interpretive Summary: Extraintestinal pathogenic Escherichia coli (ExPEC) are common contaminants in foods. Once ingested ExPEC are able to colonize the gastrointestinal (GI) tract. Once in the GI tract they can infect ulcers in the GI tract (ulcerative colitis), escape, and eventually cause sepsis (blood poisoning). In addition, the ExPEC can cause urinary tract infections, especially in women, when contaminated feces are accidentally transferred from the anus to the urethra. Genomics is now considered an integral part of microbial risk assessments for food safety. In this study we report the genomic DNA sequence of six ExPEC isolates which includes important characteristics such as their virulence factors and antibiotic resistances. This study data will allow improved risk assessments which will provide safer foods for consumers.

Technical Abstract: Potential Extraintestinal pathogenic Escherichia coli isolates DP254, WH333, WH398, F356, FEX675 and FEX725 were isolated from retail chicken meat products. Here, we report the draft genome sequences for these six E. coli isolates, which are currently being used in food safety research.