|Northrop, Emmalee - South Dakota State University|
|Rich, Jerica - South Dakota State University|
|Cushman, Robert - Bob|
|Ge, Xijin - South Dakota State University|
|Perry, George - South Dakota State University|
Submitted to: Animal Reproduction
Publication Type: Abstract Only
Publication Acceptance Date: 6/18/2018
Publication Date: 9/4/2018
Citation: Northrop, E., Rich, J., Cushman, R., Ge, X., Perry, G. 2018. Influence of preovulatory estradiol on uterine luminal fluid proteomics around maternal recognition of pregnancy in beef cattle [abstract]. Animal Reproduction. 15(Supplement 1):1065.
Technical Abstract: Proteins within the uterine luminal fluid (ULF) are involved in elongation, recognition of pregnancy, implantation, and placentation, and previous research has established that elevated preovulatory estradiol increased embryo survival and pregnancy rates. However, on d 16 of pregnancy no differences in conceptus recovery rates or IFNT concentrations were detected between animals with elevated or low concentrations of estradiol prebreeding. The present study evaluated the effects of preovulatory estradiol and conceptus presence on the d 16 ULF proteome. Beef cows/heifers (n=29) were synchronized and artificially inseminated (d 0), and grouped into high (highE2) and low (lowE2) preovulatory estradiol based on expression of estrus and circulating estradiol concentrations (d-2 and d0). On d 16, animals were slaughtered and uteri were flushed. Two separate ULF pools were created based on the following groupings: highE2/no conceptus, highE2/conceptus, lowE2/no conceptus, and lowE2/conceptus. Pools were then analyzed using a 2D LC-MS/MS based 8plex iTRAQ quantitative method. Scaffold Q+ was used to quantitate peptide and protein identifications. Peptide identifications were accepted if they could be established at greater than 95% probability by Scaffold Local FDR algorithm. Protein identifications were accepted if they could be established at greater than 99% probability and contained at least 2 identified peptides. The FDR was adjusted using Benjamini-Hochberg procedure to identify significance (P < 0.05) based on permutation tests. String database was used to analyze upregulated and downregulated proteins in each grouping. There was a total of 6989 peptides and 1269 proteins identified, and a total of 48 significant differentially expressed proteins between the highE2/conceptus group and the lowE2/conceptus group, 19 of which were upregulated (GOT2, ACAA1, TSTD1, GPLD1, ALDH2) and 29 were downregulated (UTMP, ORM1, ANXA8, ANXA1, FGG) in the highE2/conceptus group. Proteins associated with cell differentiation, single organism process, and regulation of metabolic process were upregulated in highE2/conceptus group. Proteins associated with angiogenesis, regulation of vesicle mediated transport, and positive regulation of transport were downregulated in highE2/conceptus group. A previous study identified 30 unique proteins produced by a short term culture of d 16 concepti, 24 were identified in the current study (20 were upregulated and 4 were downregulated in the highE2/conceptus group). These data provide insight on differences in specific protein abundances in the ULF that may contribute to improved conceptus survival among highE2 animals.