Location: Vegetable Crops ResearchTitle: Testing an efficient method for dihaploid potato germplasm development
Submitted to: Meeting Abstract
Publication Type: Abstract Only
Publication Acceptance Date: 3/19/2018
Publication Date: 7/22/2018
Citation: Busse, J.S., Jansky, S.H., Bethke, P.C. 2018. Testing an efficient method for dihaploid potato germplasm development. Meeting Abstract. 96:322. https://doi.org/10.1007/s12230-019-09723-w.
Technical Abstract: Potato cultivar improvement is a slow process owing in part to tetraploid genetics. We are interested in developing diploid potato breeding lines as a tool for increasing the efficiency of cultivar development. We are reporting on a large scale dihaploid line production method tested during the summer of 2017. Approximately 400 Red Norland potato stems were collected from a commercial field. Stems with only the first flower opening were collected. Stems were placed in glass bottles filled with tap water. A group of seven people emasculated and pollinated 1200 flowers in three hours. The bottles with stems were placed in a room at the University of Wisconsin-Madison Biotron controlled environment facility. The room was maintained at 20C for twenty four hours and reduced to 18C after that with a 24 hour photoperiod. Three weeks later, we collected 1007 berries, of which 378 were parthenocarpic and 199 were rotten due to unknown causes. In the remaining berries, 90 seeds with the embryo spot marker were extracted and discarded, while 181 seeds lacking the marker were retained. These seeds were treated with gibberellic acid and sown. Only 12 vigorous plants grew, and nine appear to be dihaploids based on chloroplast per guard cell counts (5-7 cp/cell). We will genotype these dihaploids and evaluate their value as parents in a diploid breeding program.