Submitted to: Journal of Animal Science
Publication Type: Abstract Only
Publication Acceptance Date: 5/6/2018
Publication Date: 12/7/2018
Citation: Li, C., Baldwin, R.L. 2018. Profiling of transcriptome responses of the intestinal epithelium to starch hydrolysate direct infusion using RNA-seq technology. Journal of Animal Science. 96(Suppl_3):425. https://doi.org/10.1093/jas/sky404.931.
Technical Abstract: Previous research has demonstrated a positive relationship between dietary MEI and increased maintenance energy costs associated with the visceral tissues. The experiments were conducted with a single nutrient (starch hydrolysate) infusion directly to the intestine to mimic changes and the next-generation sequencing technology (RNA-Seq) was used for profiling and comparison of the transcriptome of cattle intestinal epithelial tissues from cattle during the time course of the starch infusion. The infusion was for seven days. Samples of intestinal epithelial tissue were collected during the infusion on the day before the infusion, and day 1, day 3 and day 7 during the infusion. Samples were also collected on day 1 and day 7 after infusion (Day 8 and Day 14 of the experiment). RNA-seq revealed significant dynamic changes of global gene expression during infusion. On day 7 of the infusion, 1490 genes were found to be significantly differentially expressed (DE) with FDR p-value < 0.05, comparing to 105 genes on day 1 and 246 genes on Day 3. After finishing infusion, however, on day 8, only 428 genes were significantly differentially expressed and only 107 genes on Day 14. These results indicated that the differential expression of genes was the direct result of the starch infusion. By performing function and pathway analysis of DE genes using IPA, we found that digestive system development and function are among the major functions of the DE genes. The major transcription regulators such as PTH, JUN, WNT, and TNFRSF11B were identified as the activated upstream regulator.