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ARS Home » Northeast Area » Ithaca, New York » Robert W. Holley Center for Agriculture & Health » Plant, Soil and Nutrition Research » Research » Publications at this Location » Publication #350643

Research Project: Bioavailability of Iron, Zinc and Select Phytochemicals for Improved Health

Location: Plant, Soil and Nutrition Research

Title: The linoleic acid: dihomo-y-linolenic acid ratio predicts the efficacy of Zn biofortified wheat in chicken (Gallus gallus)

Author
item Tako, Elad
item KNEZ, MARIJA - Flinders University
item Kolba, Nikolai
item Glahn, Raymond
item IRELAND, EMMA - Flinders University
item STANGOULIS, JAMES - Flinders University

Submitted to: Journal of Agricultural and Food Chemistry
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 1/23/2018
Publication Date: 1/23/2018
Citation: Tako, E.N., Knez, M., Kolba, N.J., Glahn, R.P., Ireland, E., Stangoulis, J. 2018. The linoleic acid: dihomo-y-linolenic acid ratio predicts the efficacy of Zn biofortified wheat in chicken (Gallus gallus). Journal of Agricultural and Food Chemistry. 66(6):1394-1400. http://doi.org/10.1021/acs.jafc.7b04905.
DOI: https://doi.org/10.1021/acs.jafc.7b04905

Interpretive Summary: The quantity of Zn absorbed from Zn enhanced wheat material using an animal model of Zn absorption has been determined. Red blood cell membrane long chain fatty acids ratio was used as a biomarker of Zn status. Two groups of chickens were fed different diets: a high Zn (46.5 ppm) and a low Zn wheat based diet (32.8 ppm). Dietary Zn intakes, body weight, blood Zn, and the red blood cell fatty acid profile were measured and tissues were collected for protein analysis. Serum Zn concentrations were significantly greater in the high Zn group. Intestinal expression of various Zn related proteins increased in the birds fed a low Zn diet. The fatty acids ratio was higher in the birds fed the low Zn diet. The higher amount of Zn in the biofortified wheat resulted in a greater Zn uptake.

Technical Abstract: The quantity of Zn absorbed from Zn biofortified wheat material using an in-vivo model of Zn absorption has been determined. Erythrocyte linoleic: dihomo -y-linolenic acid (LA: DGLA) ratio was used as a biomarker of Zn status. Two groups of chickens (n = 15) were fed different diets: a high Zn (46.5 ug Zn g-1) and a low Zn wheat based diet (32.8 ug Zn g-1). Dietary Zn intakes, body weight, serum Zn, and the erythrocyte fatty acid profile were measured and tissues were collected for gene expression analysis. Serum Zn concentrations were greater in the high Zn group (p<0.05). Duodenal mRNA expression of various Zn transporters demonstrated expression upregulation in the birds fed a low Zn diet (n=15, p<0.05). The LA: DGLA ratio was higher in the birds fed the low Zn diet (p<0.05). The higher amount of Zn in the biofortified wheat resulted in a greater Zn uptake.