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ARS Home » Northeast Area » Washington, D.C. » National Arboretum » Floral and Nursery Plants Research » Research » Publications at this Location » Publication #350185

Research Project: Detection, Identification, and Characterization of New and Emerging Viral and Bacterial Diseases of Ornamental Plants

Location: Floral and Nursery Plants Research

Title: Sequencing, genome analysis and host range of a novel Ralstonia phage RsoP1EGY isolated from Egypt

item Ebrahim, Abdelmonian Ali - Minia University
item Elhalag, Kamel - Agricultural Research Center Of Egypt
item Addy, Hardian Susilo - University Of Jember
item Hussien, Ahmed - Agricultural Research Center Of Egypt
item Nasr-eldin, Mohamed - Benha University
item Huang, Qi

Submitted to: Archives of Virology
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 3/31/2018
Publication Date: 4/13/2018
Citation: Ebrahim, A., Elhalag, K.M., Addy, H., Hussien, A.S., Nasr-Eldin, M.A., Huang, Q. 2018. Sequencing, genome analysis and host range of a novel Ralstonia phage RsoP1EGY isolated from Egypt. Archives of Virology.

Interpretive Summary: A bacterium causes a serious brown rot disease in potato and is not allowed to enter the U. S., Canada, Europe, and Egypt. We found a virus in the soil of a potato field in Egypt that can kill the bacterium. We purified and characterized the virus, and determined its nucleotide and amino acid sequences. Our study is important for a better understanding of the virus and for developing effective control and specific diagnostic methods for this important bacterium.

Technical Abstract: A novel Ralstonia phage was isolated from soil in Egypt and designated RsPod1SoilEGY using our new four-part phage identifier naming system. When tested, this phage selectively infected only race 3 biovar 2 phylotype IIB sequevar 1, and not non-race 3 biovar 2 strains of Ralstonia solanacearum. The phage has an icosahedral capsid of 60 ± 5 nm in diameter with a short tail of 15 ± 5 nm in length, typical of a podovirus. The genome of RsPod1SoilEGY is 41,297-bp in size containing 50 open reading frames, with no significant sequence identity to any other reported R. solanacearum or non-Ralstonia phages, except a recently deposited but unreported and unclassified “Ralstonia phage DU_RP_I”. RsPod1SoilEGY is the first sequenced and characterized R. solanacearum phage isolated from Egypt.