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ARS Home » Plains Area » Manhattan, Kansas » Center for Grain and Animal Health Research » Stored Product Insect and Engineering Research » Research » Publications at this Location » Publication #349984

Research Project: Sustainable Management Strategies for Stored-Product Insects

Location: Stored Product Insect and Engineering Research

Title: Analysis of cross-resistance to Vip3 proteins in eight insect colonies, from four insect species, selected for resistance to Bacillus thuringiensis insecticidal proteins

Author
item GOMIS-CEBOLLA, JOAQUIN - University Of Valencia
item WANG, YUEQUIN - Chinese Academy Of Agricultural Sciences
item QUAN, YUDONG - University Of Valencia
item HE, KANGLAI - Chinese Academy Of Agricultural Sciences
item WALSH, TOM - Cornell University - New York
item JAMES, BILL - Csiro, Black Mountain Laboratories
item DOWNES, SHARON - Csiro, Australian Cotton Research Institute, Narrabri
item KAIN, WENDY - Csiro, Australian Cotton Research Institute, Narrabri
item WANG, PING - Cornell University - New York
item Leonard, Kathy
item Morgan, Thomas
item Oppert, Brenda
item FERRE, JUAN - University Of Valencia

Submitted to: Journal of Invertebrate Pathology
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 5/14/2018
Publication Date: 5/16/2018
Citation: Gomis-Cebolla, J., Wang, Y., Quan, Y., He, K., Walsh, T., James, B., Downes, S., Kain, W., Wang, P., Leonard, K.H., Morgan, T.D., Oppert, B.S., Ferre, J. 2018. Analysis of cross-resistance to Vip3 proteins in eight insect colonies, from four insect species, selected for resistance to Bacillus thuringiensis insecticidal proteins. Journal of Invertebrate Pathology. 155:64-70. https://doi.org/10.1016/j.jip.2018.05.004.
DOI: https://doi.org/10.1016/j.jip.2018.05.004

Interpretive Summary: The proteins called “Vip3” are specific kinds of vegetative proteins made by the bacterium Bacillus thuringiensis. We compared the effect of Vip3 proteins on a number of different insects that are susceptible or resistant to another kind of bacterial protein called “Cry” (or crystal) proteins. We found that most insect species did not demonstrate cross resistance to Vip3 and Cry toxins. However, a Cry-resistant strain of one insect, the cotton bollworm, also was resistant to Vip3 toxins. These data help producers to identify control strategies that will be effective for field pests.

Technical Abstract: Bacillus thuringiensis Vip3 proteins are synthesized and secreted during the vegetative growth phase. They are activated by gut proteases, recognize and bind to midgut receptors, form pores and lyse cells. We tested the susceptibility to Vip3Aa and Vip3Ca of Cry1A-, Cry2A-, Dipel- and Vip3-resistant insect colonies from different species to determine whether resistance to other insecticidal proteins confers cross-resistance to Vip3 proteins. As expected, the colonies resistant to Cry1A proteins, Dipel (Helicoverpa armigera, Trichoplusia ni, Ostrinia furnacalis and Plodia interpunctella) or Cry2Ab (H. armigera and T. ni) were not cross-resistant toVip3 proteins. In contrast, H. armigera colonies resistant to Vip3Aa or Vip3Aa/Cry2Ab showed cross-resistance to the Vip3Ca protein. Moreover, the Vip3Ca protein was highly toxic to O. furnacalis (LC50 not significantly different from that of Cry1Ab), whereas the Vip3Aa protein only showed moderate growth inhibition at the highest concentration tested (100 µg/g of diet). These results extend the cross-resistance studies between Vip3 and Cry proteins, show for the first time cross-resistance between proteins within the Vip3 subfamily, and points to O. furnacalis as a target for the Vip3Ca protein.