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ARS Home » Midwest Area » West Lafayette, Indiana » Livestock Behavior Research » Research » Publications at this Location » Publication #349289

Research Project: Protecting the Welfare of Food Producing Animals

Location: Livestock Behavior Research

Title: Probiotic microbes evoke different responses from bovine BAL in vitro

Author
item Eicher, Susan
item Chitko Mckown, Carol
item Bryan, Kieth - Chr Hansen Inc

Submitted to: American Association of Immunologists Proceedings
Publication Type: Abstract Only
Publication Acceptance Date: 3/16/2018
Publication Date: 5/4/2018
Citation: Eicher, S.D., Chitko-McKown, C.G., Bryan, K. 2018. Probiotic microbes evoke different responses from bovine BAL in vitro. The Journal of Immunology. 200(1):59.21.

Interpretive Summary:

Technical Abstract: Probiotics are used for enteric health, but how they interact with upper respiratory immune cells is not known. We aimed to determine how probiotics affect functions and markers of bronchoalveolar lung lavage cells (BAL). BAL were isolated from lungs of calves at slaughter. Bacteria treatments were a Lactobacillus probiotic (US), a commercially available probiotic (PB), Lactobacillus animalis (LA-51), Propionibacterium freudenreichii (PF-24), Enterococcus faecium (CH-212), E. faecium (SF-273), E. faecium (M-74), Bifidobacterium animalis ssp lactis (BB-12), Bacillus subtilis (EB-15 ), and Bacillus amyloliquifaciens (ZM-16). Microbial cells and BAL were incubated 1:1 for 1 h at 37° C and 5 % CO2, then nothing (control, CNT), CD14 (FITC) and CD205 (RPE), CD18 (Alexa Fluor 647), and E. coli bioparticles (Molecular Probes) were added. Reported differences are P = 0.05. Mean fluorescence of phagocytosis of E. coli bioparticles was less than CNT for BB-12 and EB-15, but percentage (%) of cells phagocytizing was not different among treatments. Oxidative burst of M-74 was less than CH-212, BB-12, EB-15, PB, and US, but none were different than CNT. The % of cells with oxidative burst was greater for PF-24 than for LA-51, BB-12, ZM-16, PB, and CNT. CD 14 mean fluorescence was least for M-74 compared with all other microbes and CNT, but % of cells expressing CD14 was greatest on PF-24 and US compared with EB-15 and BB-12, but not CNT. DC-205 mean fluorescence was greatest for M-74 compared with LA-51, PF-24, CH-212, SF-273, CNT, and US. Percentage of cells expressing DC-205 and CD18 was not different from controls. PF-24 and M-74 were most immunomodulatory compared with controls. M-74 appears to be suppressive and PF-24 increases leukocyte functions, showing that probiotic bacteria differentially modulate BAL. Knowing these interactions can help in selection of microbes to use in targeted probiotic production.