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ARS Home » Pacific West Area » Logan, Utah » Poisonous Plant Research » Research » Publications at this Location » Publication #348819

Research Project: Understanding and Mitigating the Adverse Effects of Poisonous Plants on Livestock Production Systems

Location: Poisonous Plant Research

Title: Genetic relationships among different chemotypes of Lupinus sulphureus

Author
item Cook, Daniel
item Mott, Ivan
item Larson, Steven
item Lee, Stephen
item JOHNSON, ROBERT - Brigham Young University
item Stonecipher, Clinton - Clint

Submitted to: Journal of Agricultural and Food Chemistry
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 1/26/2018
Publication Date: 2/13/2018
Citation: Cook, D., Mott, I.W., Larson, S.R., Lee, S.T., Johnson, R., Stonecipher, C.A. 2018. Genetic relationships among different chemotypes of Lupinus sulphureus. Journal of Agricultural and Food Chemistry. 66(8):1773-1783. https://doi.org/10.1021/acs.jafc.7b05884.
DOI: https://doi.org/10.1021/acs.jafc.7b05884

Interpretive Summary: Lupines (Lupinus spp.) are a common plant legume species found on western U.S. rangelands. Lupinus spp. may contain quinolizidine and or piperidine alkaloids that can be toxic and or teratogenic to grazing livestock. Alkaloid profiles may vary between and within a species. The objectives of this study were to 1) further explore the characteristic alkaloid profiles of L. sulphureus using field collections and 2) to explore the phylogenetic relationship of the different populations and chemotypes of L. sulphureus using AFLP method of DNA fingerprinting, thus providing possible explanations to the phenomena of multiple chemotypes within a species. Forty-nine accessions of L. sulphureus were classified into 7 chemotypes. The DNA profiles showed that one L. sulphureus chemotype, chemotype A, is genetically divergent from the other chemotypes of L. sulphureus, suggesting it represents an unresolved lupine taxon, possibly a new lupine species. Additionally, the different chemotypes of L. sulphureus chemotypes represented different genetic groups as shown by Bayesian cluster analysis and principle component analysis.

Technical Abstract: Lupines (Lupinus spp.) are a common plant legume species found on western U.S. rangelands. Lupinus spp. may contain quinolizidine and or piperidine alkaloids that can be toxic and or teratogenic to grazing livestock. Alkaloid profiles may vary between and within a species. The objectives of this study were to 1) further explore the characteristic alkaloid profiles of L. sulphureus using field collections and 2) to explore the phylogenetic relationship of the different populations and chemotypes of L. sulphureus using AFLP method of DNA fingerprinting, thus providing possible explanations to the phenomena of multiple chemotypes within a species. Forty-nine accessions of L. sulphureus were classified into 7 chemotypes. The DNA profiles showed that one L. sulphureus chemotype, chemotype A, is genetically divergent from the other chemotypes of L. sulphureus, suggesting it represents an unresolved lupine taxon, possibly a new lupine species. Additionally, the different chemotypes of L. sulphureus chemotypes represented different genetic groups as shown by Bayesian cluster analysis and principle component analysis.