|GEISER, DAVID - Pennsylvania State University|
|RHEEDER, JOHN - Cape Peninsula University Of Technology|
|HANNEKE, ALBERTS - Cape Peninsula University Of Technology|
Submitted to: Meeting Abstract
Publication Type: Abstract Only
Publication Acceptance Date: 4/11/2018
Publication Date: N/A
Technical Abstract: Fusarium ranks as one of the world's most economically destructive and taxonomically challenging genera of mycotoxigenic plant pathogens. Due to the significant threat that fusarial toxins pose to agricultural biosecurity, food safety, and plant and animal health, several compendia have attempted to catalog the diversity of toxigenic fusaria and the toxins they produce. Marasas’ et al. (1984) compendium occupies a special place in the Fusarium mycotoxicology literature because the authors tested a number of the strains in this treatise for toxins, and because the strains are archived in the South African Agricultural Research Council (ARC; formerly Medical Research Council - MRC) and Fusarium Research Center (FRC) Culture Collections. Given the transformative impact genealogical concordance phylogenetic species recognition (GCPSR)-based studies have had on Fusarium systematics over the past two decades (reviewed in Aoki et al., 2014), we initiated the present study to: (i) reevaluate the species identity and phylogenetic diversity of 156 MRC strains via GCPSR, (ii) predict mycotoxin potential of any putatively novel Fusarium species discovered within the MRC collection by mining their whole genomes for biosynthetic pathways that encode mycotoxins, and (iii) test the MRC strains for mycotoxin production in solid grain cultures and liquid media using high performance liquid chromatography-mass spectrometry and gas chromatography-mass spectrometry.