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Title: PCR assay for Verticillium dahliae race 1 resistance in lettuce based on genome sequencing of 61 accessions

item INDERBITZIN, PATRIK - University Of California
item REYES-CHIN WO, SEBASTIAN - University Of California
item CHRISTOPOULOU, MARILENA - University Of California
item MICHELMORE, RICHARD - University Of California
item SUBBARAO, KRISHNA - University Of California
item Simko, Ivan

Submitted to: International Congress of Plant Pathology Abstracts and Proceedings
Publication Type: Abstract Only
Publication Acceptance Date: 12/8/2017
Publication Date: 8/3/2018
Citation: Inderbitzin, P., Reyes-Chin Wo, S., Christopoulou, M., Michelmore, R.W., Subbarao, K.V., Simko, I. 2018. PCR assay for Verticillium dahliae race 1 resistance in lettuce based on genome sequencing of 61 accessions. International Congress of Plant Pathology, July 29-August 3, 2018, Boston, Massachusetts.

Interpretive Summary:

Technical Abstract: Verticillium dahliae is a soilborne plant pathogen that is a major threat to lettuce production in the Salinas and Pajaro Valleys of central coastal California. In tomato, resistance to V. dahliae race 1 is conferred by Ve1, which encodes a nucleotide binding-leucine rich repeat receptor (NLR) protein. There are two copies of Ve in tomato, Ve1 and Ve2, but only Ve1 provides resistance to V. dahliae. In the V. dahliae race 1-resistant lettuce cultivar La Brillante, there are three Ve homologs, Verticillium resistance 1 (Vr1), Vr2 and Vr3, which are 54 - 57% similar to Ve1 in tomato. Three Vr paralogs are also present in the V. dahliae race 1-susceptible cultivar Salinas; it is currently unknown which of the Vr paralogs confers resistance to V. dahliae race 1 in lettuce. To develop a PCR assay to detect V. dahliae race 1 resistance in lettuce, we sequenced the genomes of 60 resistant or susceptible lettuce cultivars and extracted the Vr gene-like sequences. The La Brillante Vr1 allele (LBVr1) was present in all resistant cultivars and absent in all susceptible cultivars. This indicated that LBVr1 is associated with resistance of lettuce to V. dahliae race 1. We designed a LBVr1-specific PCR assay and validated its accuracy on an additional 90 accessions. This work indicates that LBVr1 may be the functional Vr allele in lettuce. Gene knock-out assays are underway to prove the function of LBVr1 in resistance to V. dahliae race 1.