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ARS Home » Southeast Area » Stuttgart, Arkansas » Dale Bumpers National Rice Research Center » Research » Publications at this Location » Publication #348689

Research Project: Using Genetic Approaches to Reduce Crop Losses in Rice Due to Biotic and Abiotic Stress

Location: Dale Bumpers National Rice Research Center

Title: Dissecting interactions of rice major and minor blast resistance genes with yield related components

item Jia, Yulin
item CHEN, XINGLONG - China Agricultural University
item LI, WENQI - Jiangsu Academy Agricultural Sciences
item WANG, XUEYAN - University Of Arkansas
item Bianco, Tracy
item Box, Heather

Submitted to: International Congress of Plant Pathology Abstracts and Proceedings
Publication Type: Abstract Only
Publication Acceptance Date: 2/8/2018
Publication Date: 7/29/2018
Citation: Jia, Y., Chen, X., Li, W., Wang, X., Bianco, T.A., Box, H.N. 2018. Dissecting interactions of rice major and minor blast resistance genes with yield related components. International Congress of Plant Pathology Abstracts and Proceedings. Boston, Massachusetts, July 29-August 3, 2018.

Interpretive Summary:

Technical Abstract: A large recombinant inbred line (RIL) mapping population and consisting of 800 individuals was developed to investigate the functional interactions of blast resistance (R) genes, Pi-ta with Pi-b, other minor blast resistance QTL, and yield related components. US long grain tropical japonica is derived from the rice varieties Cybonnet (CYBT) and Saber (SB). CYBT is known to contain Pi-ta (avirulent on blast races, IB1, IB17, and IB49 and virulent on IE1K) and Pi-km (virulent on IB1, IB17, IB49, and IE1k) and SB has Pi-b (avirulent on IB1, IB49, IE1k and partial avirulent on IB49). The entire population was evaluated with Pi-ta and Pi-b using gene specific PCR, and with their differential blast isolates/races under greenhouse conditions. A subset of this population (243 RILs) was used to construct a linkage map with 186 single nucleotide polymorphism (SNP) markers for mapping the blast R/QTL, and QTL for 1000 grain weight, number of branches of panicle, panicle length, and weight. A total of seven blast resistance QTL’s were mapped on chromosomes 2, 3, 8, 9, 10, 11, and 12. Among them, qBR12 (Q1) on chromosome 12 was mapped at the Pi-ta locus which accounted for 28.64 to 53.46% of disease reactions, whereas qBR2 (Q2) was located at the Pi-b locus and accounted for 16.85 to 78.37% of disease reactions. There was a positive additive-by-additive epistatic interaction between Pi-ta and Pi-b suggesting that Pi-ta interacts positively with Pi-b in triggering a robust defense response. The remaining five minor resistance QTL’s have also been identified in other studies with different mapping parents suggesting that these five resistance QTL’s have coevolved and became domesticated. Relationships of the resistance effects of blast R genes/QTL with yield related components will be presented.