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ARS Home » Pacific West Area » Corvallis, Oregon » Horticultural Crops Research Unit » Research » Publications at this Location » Publication #348414
Title: Virus survey in caneberry nursery plants in Oregon

Author
item SHARMA-POUDYAL, D - Oregon Department Of Agriculture
item LANE, S - Oregon Department Of Agriculture
item GRANT, J - Oregon Department Of Agriculture
item Martin, Robert

Submitted to: Phytopathology
Publication Type: Abstract Only
Publication Acceptance Date: 3/2/2018
Publication Date: 4/9/2018
Citation: Sharma-Poudyal, D., Lane, S., Grant, J., Martin, R.R. 2018. Virus survey in caneberry nursery plants in Oregon. Phytopathology. Abstract for the International Congress of Plant Pathology, 7/29/18-8/3/18, Boston, MA.

Interpretive Summary:

Technical Abstract: Oregon is a major producer of caneberry (Rubus spp., blackberry and raspberry) nursery stock in the United States. Planting virus infected material can reduce berry yield, profitability, and the life of the berry planting. Three Oregon caneberry nurseries (Nursery A, B, and C), with different production systems, were surveyed for five economically important viruses, Black raspberry necrosis virus (BRNV), Raspberry bushy dwarf virus (RBDV), Raspberry leaf mottle virus (RLMV), Strawberry necrotic shock virus (SNSV), and Tomato ringspot virus (ToRSV). Nursery A grew micro-propagated plantlets in soilless media in a screen house; Nursery B grew plants from tissue culture and certified root cuttings in soilless media in containers in a field with gravel layer (canyard); and Nursery C grew plants from field-grown canes, suckers, and plantlets in field. Fifty-nine samples (5 leaves/sample) were collected from each nursery. RBDV, SNSV and ToRSV were tested by ELISA and BRNV and RLMV were tested by virus specific reverse transcriptase PCR. All samples tested negative for BRNV, SNSV, and ToRSV. Samples from Nursery A and B tested negative for RLMV and RBDV. Samples obtained from Nursery C tested positive 15% and 42% for RLMV and RBDV, respectively. Since virus infected nursery stocks are incurable, these survey results reinforce the importance of caneberry certification standards based on virus testing and best management practices to produce clean nursery stock for caneberry field establishment.