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ARS Home » Pacific West Area » Parlier, California » San Joaquin Valley Agricultural Sciences Center » Crop Diseases, Pests and Genetics Research » Research » Publications at this Location » Publication #348300

Research Project: Identification of Novel Management Strategies for Key Pests and Pathogens of Grapevine with Emphasis on the Xylella Fastidiosa Pathosystem

Location: Crop Diseases, Pests and Genetics Research

Title: Temperature dependent RNA metabolism in Xylella fastidiosa during cold stress and grapevine infection

item Burbank, Lindsey

Submitted to: International Congress of Plant Pathology Abstracts and Proceedings
Publication Type: Abstract Only
Publication Acceptance Date: 3/27/2018
Publication Date: N/A
Citation: N/A

Interpretive Summary:

Technical Abstract: Re-occurrence of Pierce’s disease of grapes, caused by Xylella fastidiosa, is known to be influenced by environmental factors, particularly cold temperatures during overwintering. Grapevines in colder regions are often cured of X. fastidiosa infection over the winter season, depending on cultivar, time of inoculation, and disease severity. The dynamics of X. fastidiosa low temperature adaption and survival during persistent infections in planta is still poorly understood. RNA metabolism is an essential part of bacterial response to low temperature, including inducible expression of RNA binding proteins, helicases, and exoribonucleases. Characterization of two X. fastidiosa RNA-binding family cold shock protein (CSP) homologs revealed that neither was cold-inducible at the transcriptional or post-transcriptional level, suggesting a diminished cold adaptation response in this pathogen. However, expression of X. fastidiosa cold response RNA helicase, srmB, is reduced in a CSP mutant ('csp1) compared with wild type X. fastidiosa, indicating a potential regulatory role of Csp1 in RNA metabolism during temperature response. Understanding the cold-adaptation process of X. fastidiosa is important as it relates to the ability of this pathogen to survive through the winter in infected plants depending on geographic location and climatic conditions.