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ARS Home » Northeast Area » Leetown, West Virginia » Cool and Cold Water Aquaculture Research » Research » Publications at this Location » Publication #347971

Research Project: Integrated Research Approaches for Improving Production Efficiency in Salmonids

Location: Cool and Cold Water Aquaculture Research

Title: Egg transcripts associated with family fertility in rainbow trout (Oncorhynchus mykiss)

item Ma, Hao
item MARTIN, KYLE - Troutlodge, Inc
item DIXON, II, DOUG - Troutlodge, Inc
item Weber, Gregory - Greg

Submitted to: Plant and Animal Genome Conference
Publication Type: Abstract Only
Publication Acceptance Date: 11/26/2017
Publication Date: 1/17/2018
Citation: Ma, H., Martin, K., Dixon, Ii, D., Weber, G.M. 2018. Egg transcripts associated with family fertility in rainbow trout (Oncorhynchus mykiss) [abstract]. Plant and Animal Genome Conference. P0257.

Interpretive Summary:

Technical Abstract: In order to identify molecular markers associated with rainbow trout egg quality, the RNAs of unfertilized eggs derived from 4 low and 10 high fertility families were sequenced using both poly(A) retention and rRNA removal kits. The results indicated that although the sequences derived from rRNA removal libraries identified about 20% more transcripts than that of poly(A) retention libraries, only one transcript was differentially expressed between the rRNA removal libraries, while 944 differentially expressed transcripts (DETs) were identified from poly(A) retention libraries under the same criteria. The 945 DETs were classified into 31 functional modules, of which 9 were related to ribosomes and 8 related to mitochondria. The other modules contained transcripts primarily involved in transcription, translation, cell division, apoptosis, and immunity. Small RNA sequencing of the low and high survival families identified 26 known and 38 novel differentially expressed miRNAs, which targeted 271 of the DETs. These results could be used to develop molecular markers to assess rainbow trout family fertility.