Location: Cereal Crops ResearchTitle: Fine mapping of a novel major QTL for Fusarium head blight resistance in the wheat line PI 277012 Author
|Zhao, Mingxia - North Dakota State University|
|Leng, Yueqiang - North Dakota State University|
|Liu, Yan - North Dakota State University|
|Xi, Pengen - North Dakota State University|
|Li, Jianjiang - North Dakota State University|
|Wang, Rui - North Dakota State University|
|Long, Yunming - North Dakota State University|
|Zhong, Shaobin - North Dakota State University|
Submitted to: Meeting Abstract
Publication Type: Abstract Only
Publication Acceptance Date: 11/8/2017
Publication Date: 12/3/2017
Citation: Zhao, M., Leng, Y., Liu, Y., Xi, P., Li, J., Wang, R., Long, Y., Chao, S., Xu, S.S., Zhong, S. 2017. Fine mapping of a novel major QTL for Fusarium head blight resistance in the wheat line PI 277012 [abstract]. In: Proceedings of the 2017 National Fusarium Head Blight Forum. December 3-5, 2017. Mulwaukee, Wisconsin. p. 98.
Technical Abstract: The hexaploid wheat line PI 277012 exhibited a high level of Fusarium head blight (FHB) resistance in both greenhouse and field experiments. Previous QTL analysis of a population consisting of 130 doubled haploid (DH) lines from a cross between PI 277012 and ‘Grandin’ identified two major FHB resistance QTLs located on chromosome arms 5AS and 5AL, respectively. The 5AS QTL (Qfhb.rwg-5A.1) peaked at marker Xbarc40 between markers Xcfa2104 and Xgwm617, while the 5AL QTL (Qfhb.rwg-5A.2) peaked at marker Xcfd39 between markers Xwmc470 and Xbarc48. Qfhb.rwg-5A.2 is different from those found in other known sources of FHB resistance. To fine map Qfhb.rwg-5A.2 with more DNA markers, the DH population (GP-DH) was first genotyped using the wheat 9K SNP iSelect assay, and a total of 4317 polymorphic SNPs between PI 277012 and Grandin were mapped to the genetic linkage map previously constructed for chromosome 5A with SSR markers. Sequences of SNP markers within the peak of Qfhb.rwg-5A.2 were used to search for syntenic region in Brachypodium distacheon (Bd) genome and a Bd region containing 2,500 genes were identified. The Bd genes were used as queries to search for the wheat genome survey sequences and 139 contigs were identified for primer design. Ten Cleaved Amplified Polymorphic Sequences (CAPS) markers were developed from the wheat contig sequences and mapped to the Qfhb.rwg-5A.2 region in the GP-DH population. Additional DNA markers were developed for the Qfhb.rwg-5A.2 region from the wheat 90K SNP iSelect assay and reference genome sequences of Chinese Spring. By using these DNA markers to genotype 947 recombinant inbred lines from the cross between PI 277012 and Grandin, we delimited Qfhb.rwg-5A.2 in a 1.09-Mbp genomic region according to the reference genome sequence of Chinese Spring. This study provides DNA markers tightly linked to Qfhb.rwg-5A.2, which can be used for marker-assisted selection and facilitate the isolation and functional characterization of the underlying resistance gene.