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ARS Home » Midwest Area » Peoria, Illinois » National Center for Agricultural Utilization Research » Mycotoxin Prevention and Applied Microbiology Research » Research » Publications at this Location » Publication #347649
Research Project: Improved Analytical Technologies for Detection of Foodborne Toxins and Their Metabolites

Location: Mycotoxin Prevention and Applied Microbiology Research

Title: Computational studies on the excited state properties of citrinin and application in fluorescence analysis

Author
item Appell, Michael
item TU, YI-SHU - National Science Council
item BOSMA, WAYNE - Bradley University

Submitted to: American Chemical Society National Meeting
Publication Type: Abstract Only
Publication Acceptance Date: 3/22/2018
Publication Date: N/A
Citation: N/A

Interpretive Summary:

Technical Abstract: Citrinin is a mycotoxin of increasing concern that is produced by fungi associated with maize, red yeast rice, and other agricultural commodities. A comprehensive time-dependent density functional study on the excited state properties of citrinin was conducted to identify parameters for reliable detection. Deprotonation of citrinin is a major factor contributing to the shifts in fluorescence excitation and emission maxima. The intrinsic fluorescence of citrinin is strongly related to structure, influenced by environment, and favored by highly acidic conditions. Based on these results, a liquid chromatography fluorescence detection method to determine levels of citrinin in maize was developed utilizing molecularly imprinted solid-phase extraction (MISPE) sample clean-up (limit of detection 0.01 microgram/gram). The time dependent density functional analysis explained the experimental fluorescence behavior and assisted in analytical method development to detect citrinin maize.