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Title: Four new species of Metschnikowia and the transfer of seven Candida species to Metschnikowia and Clavispora as new combinations

item Kurtzman, Cletus
item ROBNETT, CHRISTIE - Retired ARS Employee
item Basehoar, Eleanor
item Ward, Todd

Submitted to: Antonie Van Leeuwenhoek
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 5/2/2018
Publication Date: 5/12/2018
Citation: Kurtzman, C.P., Robnett, C.J., Basehoar, E., Ward, T.J. 2018. Four new species of Metschnikowia and the transfer of seven Candida species to Metschnikowia and Clavispora as new combinations. Antonie Van Leeuwenhoek.

Interpretive Summary: Yeasts such as Metschnikowia pulcherrima and M. fructicola have proven effective for biocontrol of rots during storage of fruit. We discovered four new species of Metschnikowia that are related to the preceding two species and may provide additional potential for control of storage rots of fruit and perhaps other crops. To facilitate exploitation of these species, we have provided formal descriptions and genetic characterization from four separate gene sequences. The new species are being described as M. peoriensis, M. rubicola, M. leonuri and M. anglica. In addition, seven species of Candida were discovered to be members of Metschnikowia and the related genus Clavispora. To satisfy requirements of the taxonomic code for classification of fungi, the seven species are being transferred to these latter two genera. In continuing work, the four new species of Metschnikowia will be made available to scientists working with prevention of fruit rots, and they will also be tested in NCAUR programs for prevention of disease in wheat.

Technical Abstract: From comparisons of ITS1-5.8S-ITS2 and gene sequences for nuclear D1/D2 LSU rRNA, nuclear SSU (18S) rRNA, translation elongation factor 1-a (EF1-a) and RNA polymerase II subunit 2 (RPB2), the following four new ascosporogenous yeast species were resolved and are described as Metschnikowia anglica (NRRL Y-7298T [type strain], CBS 15342, MycoBank MB 823167), Metschnikowia leonuri (NRRL Y-6546T, CBS 15341, MB 823166), Metschnikowia peoriensis (NRRL Y-5942T, CBS 15345, MB 823164) and Metschnikowia rubicola (NRRL Y-6064T, CBS 15344, MB 823165). The following six species of Candida are members of the Metschnikowia clade and are proposed for transfer to Metschnikowia as new combinations: Candida chrysomelidarum (NRRL Y-27749T, CBS 9904, MB 823223), Candida gelsemii (NRRL Y-48212T, CBS 10509, MB 823192), Candida kofuensis (NRRL Y-27226T, CBS 8058, MB 823195), Candida picachoensis (NRRL Y-27607T, CBS 9804, MB 823197), Candida pimensis (NRRL Y-27619T, CBS 9805, MB 823205) and Candida rancensis (NRRL Y-48702T, CBS 8174, MB 823224). Candida fructus (NRRL Y-17072T, CBS 6380, MB 823206) is transferred to Clavispora as a new combination, and Candida musae is shown to be a synonym of C. fructus. Apparent multiple alleles for ITS, D1/D2, EF1-a and RPB2 were detected in strains of some species.