Skip to main content
ARS Home » Midwest Area » East Lansing, Michigan » Sugarbeet and Bean Research » Research » Publications at this Location » Publication #347132

Research Project: Genetic Enhancement of Dry Bean Nutritional and Processing Qualities

Location: Sugarbeet and Bean Research

Title: Meta-QTL analysis of seed iron and zinc concentration in common bean (Phaseolus vulgaris L.)

Author
item Izqierdo, Paulo - Michigan State University
item Astudillo, Carolina - Michigan State University
item Iqbal, Asif - Michigan State University
item Blair, Matthew - Tennessee State University
item Raatz, Bodo - International Center For Tropical Agriculture (CIAT)
item Cichy, Karen

Submitted to: Theoretical and Applied Genetics
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 5/2/2018
Publication Date: 5/11/2018
Citation: Izqierdo, P., Astudillo, C., Iqbal, A., Blair, M., Raatz, B., Cichy, K.A. 2018. Meta-QTL analysis of seed iron and zinc concentration in common bean (Phaseolus vulgaris L.). Theoretical and Applied Genetics. https://doi.org/10.1007/s00122-018-3104-8.
DOI: https://doi.org/10.1007/s00122-018-3104-8

Interpretive Summary: Micronutrient deficiencies of iron and zinc are major nutritional disorders affecting billions of people around the world. Dry beans (Phaseolus vulgaris L.) biofortification programs are a means to address human micronutrient deficiencies by developing bean cultivars with increased levels of these elements. Biofortification efforts thus far have been conducted by phenotypic screening for seed Fe and Zn. While numerous quantitative trait loci (QTL) studies have been conducted to identify genomic regions associated with increased Fe and Zn concentration in seeds, these results have yet to be employed for marker assisted breeding. We conducted a meta-analysis from seven QTL studies to identify the regions in the bean genome that control seed Fe and Zn concentration. In total, four Meta QTL specific to Fe and three Meta QTL specific to Zn were identified. Additionally, eight Meta QTL that co-localized for Fe and Zn concentration were identified across 8 chromosomes. These Meta QTL that co-localize for Fe and Zn could be useful candidates for marker assisted breeding to simultaneously increase the concentration of both micronutrients.

Technical Abstract: Common bean (Phaseolus vulgaris L.) is the most important legume for human consumption worldwide and it is an important source of microelements, especially iron and zinc. Bean biofortification programs develop new varieties with high levels of Fe and Zn targeted for countries with human micronutrient deficiencies. Biofortification efforts thus far have relied on phenotypic selection of raw seed mineral concentrations in advanced generations. While numerous quantitative trait loci (QTL) studies have been conducted to identify genomic regions associated with increased Fe and Zn concentration in seeds, these results have yet to be employed for marker assisted breeding. The objective of this study was to conduct a meta-analysis from seven QTL studies in Andean and Middle American intra and inter genepool populations to identify the regions in the genome that control the Fe and Zn levels in seeds. In total, four Meta QTL specific to Fe and three Meta QTL specific to Zn were identified. Additionally, eight Meta QTL that co-localized for Fe and Zn concentration were identified across eight chromosomes. Individually 10 to 27% of the phenotypic variation was explained by the shared Meta QTL. They could be useful candidates for marker assisted breeding to simultaneously increase seed Fe and Zn. The physical positions for 15 individual Meta-QTL were identified across six recombinant inbred bi-parental and one advanced backcross population. In the 15 Meta-QTLs we identified 15 candidate genes that belong to seven gene families that have been related with transport of iron and zinc in plants.