Complete genome sequences of two Mycoplasma gallisepticum F-strain variants

Authors: Leigh, Spencer; Evans, Jeffrey - Jeff; Branton, Scott

Submitted to: American Society for Microbiology
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 7/29/2019
Publication Date: 8/15/2019
Citation: Leigh, S.A., Evans, J.D., Branton, S.L. 2019. Complete genome sequences of two Mycoplasma gallisepticum F-strain variants. American Society for Microbiology. 8:e00485-19. https://doi.org/10.1128/mra.00485-19.
DOI: https://doi.org/10.1128/mra.00485-19

Interpretive Summary: Multiple Mycoplasma gallisepticum F-strain vaccines are being marketed to poultry producers. Research has demonstrated that significant differences in the host immune response to the vaccine can be obtained when chickens are vaccinated with fractional vaccine doses, suggesting potentially significant differences exist between the various vaccines. Whole genome sequencing was performed to identify genetic differences between the vaccines. Analysis of the sequencing results identified a small number of genetic differences between the strains, including significant differences in two genes and other minor genetic differences that could also impact the host immune response. These results can be used to further understand that role that expressed M. gallisepticum proteins have on the host immune response.

Technical Abstract: Mycoplasma gallisepticum, represented by two commercial vaccines and one lab strain precursor, has proven to be an effective vaccine for preventing M. gallisepticum induced pathology. Studies comparing the strains have noted differences in host serological response. Genomic sequencing and analysis were performed to identify potential mutations causing the difference in host immune response. Although no single change could be identified as causing the difference, potential changes in vlhA gene expression and a premature stop codon in another gene may be responsible for the difference in host immune response.