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ARS Home » Pacific West Area » Pullman, Washington » WHGQ » Research » Publications at this Location » Publication #346384

Research Project: Genetic Improvement of Wheat and Barley for Resistance to Biotic and Abiotic Stresses

Location: Wheat Health, Genetics, and Quality Research

Title: Characterizing and validating stripe rust resistance loci in US Pacific Northwest winter wheat accessions (Triticum aestivum L.) by genome-wide association and linkage mapping

Author
item LIU, WEIZHEN - Washington State University
item NARUOKA, YUKIKO - Syngenta
item MILLER, KAITLIN - Washington State University
item Garland-Campbell, Kimberly
item CARTER, ARRON - Washington State University

Submitted to: The Plant Genome
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 11/9/2017
Publication Date: 2/8/2018
Citation: Liu, W., Naruoka, Y., Miller, K., Garland Campbell, K.A., Carter, A.H. 2018. Characterizing and validating stripe rust resistance loci in US Pacific Northwest winter wheat accessions (Triticum aestivum L.) by genome-wide association and linkage mapping. The Plant Genome. https://doi.org/10.3835/plantgenome2017.10.0087.
DOI: https://doi.org/10.3835/plantgenome2017.10.0087

Interpretive Summary: Stripe rust is a major global disease of wheat affecting millions of hectares each year. In the Pacific Northwest, stripe rust resistance is a requirement for newly released cultivars and the Washington State and USDA breeding programs have incorporated genes for resistance for many years. This research examined the location and number of genes associated with resistance to stripe rust using four populations of soft white winter wheat that are adapted to the Pacific Northwest. Stripe rust was rated for field resistance in ten environments and for seedling resistance using multiple races of stripe rust. Major loci were identified that carried resistance on chromosome 1B, and 2A and seven additional loci were identified that contributed to the level or resistance. These results will enable wheat breeders to incorporate multiple genes for resistance to stripe rust using molecular marker assisted breeding and to reduce the threat of this disease and extend the utility of genes for resistance.

Technical Abstract: Stripe rust resistance is a critical need for wheat cultivars in the US Pacific Northwest (PNW). Our previous genome-wide association study (GWAS) for stripe rust resistance in a set of PNW winter wheat accessions (Panel-2) identified multiple QTL for both all-stage and field resistance. In this study, we conducted additional GWAS using a different set of PNW winter wheat accessions (Panel-1) that contained recently bred soft white winter wheat breeding lines and cultivars. A total of 12 all-stage resistance QTL and eight field resistance QTL were identified. Within these QTL, nine QTL for all-stage resistance QTL and two QTL for field resistance were located distinctly from previously characterized genes and likely represent novel loci. QYr.wac-1B.1, QYr.wac-2A, and QYr.wac-2B explained the largest phenotypic variances for disease response. The analysis confirmed that Qyr.wac-1B.1 was indeed the same as Qyr.wpg-1B.2 identified in Panel-2 and that QYr.wac-2A was likely Yr17 and closely linked to another field resistance QTL, QYr.wpg-2A.2 (Panel-2). Haplotypes for Qyr.wac-1B.1 and for Yr17 and linked loci on chromosome 2A provide practical information for marker development and introgression of these QTL into wheat breeding programs.