Location: National Peanut Research LaboratoryTitle: Linkage analysis and QTL mapping in a tetraploid russet mapping population of potato
|MANRIQUE-CARPINTERO, NORMA - Michigan State University|
|COOMBS, JOSEPH - Michigan State University|
|YENCHO, CRAIG - North Carolina State University|
|BRANDT, TINA - Jr Simplot Company|
|GUPTA, SANJAY - University Of Minnesota|
|Novy, Richard - Rich|
|DOUCHES, DAVID - Michigan State University|
Submitted to: BioMed Central (BMC) Genetics
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 9/12/2018
Publication Date: 9/21/2018
Citation: Massa, A.N., Manrique-Carpintero, N.C., Coombs, J.J., Haynes, K.G., Bethke, P.C., Yencho, C.G., Brandt, T., Gupta, S.K., Novy, R.G., Douches, D.S. 2018. Linkage analysis and QTL mapping in a tetraploid russet mapping population of potato. BioMed Central (BMC) Genetics. 19:87. https://doi.org/10.1186/s12863-018-0672-1.
Interpretive Summary: The objective of this research was to identify quantitative trait loci (QTLs) in a tetraploid F1 mapping population derived from a cross between ‘Rio Grande Russet’ × ‘Premier Russet’; two varieties representative of the U.S. market class characterized by long tubers with russet skin suitable for use in processing or fresh consumption. Six important regions on five potato chromosomes (4, 5, 6, 10, 11) were associated with tuber sugar concentration and tuber processing quality. Novel molecular markers with a high potential for the improvement of tuber processing quality at the tetraploid level, especially within the russet market class represented by the parents of the mapping population were identified. The ability to process into French fries with the light color desired by consumers is a key attribute within the russet market class.
Technical Abstract: Genome-wide single nucleotide polymorphism (SNP) markers coupled with allele dosage information has emerged as a powerful tool for studying complex traits in cultivated autotetraploid potato (Solanum tuberosum L., 2n'='4×'='48). To date, this approach has been effectively applied to the identification of quantitative trait loci (QTLs) underlying highly heritable traits such as disease resistance, but largely unexplored for traits with complex patterns of inheritance. In this study, an F1 tetraploid russet mapping population (162 individuals) was evaluated for multiple quantitative traits over two years and two locations to identify QTLs associated with tuber sugar concentration, processing quality, vine maturity, and other high-value agronomic traits. We report the linkage maps for the 12 potato chromosomes and the QTL location with corresponding genetic models and candidate SNPs explaining the highest phenotypic variation for tuber quality and maturity related traits. Significant QTLs for tuber glucose concentration and tuber fry color were detected on chromosomes 4, 5, 6, 10, and 11. Collectively, these QTLs explained between 24 and 46% of the total phenotypic variation for tuber glucose and fry color, respectively. The QTL on chromosome 10 was associated with apoplastic invertases, with ‘Premier Russet’ contributing the favorable allele for fry processing quality. On chromosome 5, minor-effect QTLs for tuber glucose concentration and fry color co-localized with various major-effect QTLs, including vine maturity, growth habit, tuber shape, early blight (Altenaria tenuis), and Verticillium wilt (Verticillium spp.). Linkage analysis and QTL mapping in a russet mapping population (A05141) using SNP dosage information successfully identified favorable alleles and candidate SNPs for resistance to the accumulation of tuber reducing sugars. These novel markers have a high potential for the improvement of tuber processing quality. Moreover, the discovery of different genetic models for traits with overlapping QTLs at the maturity locus clearly suggests an independent genetic control.