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ARS Home » Midwest Area » East Lansing, Michigan » Sugarbeet and Bean Research » Research » Publications at this Location » Publication #345669
Title: A SNP mutation affects rhizomania-virus content of sugar beets grown on resistance-breaking soi

Author
item BROCCANELLO, CHIARA - Universita Di Padova
item McGrath, Jon
item Panella, Leonard
item Richardson, Kelley
item CHIODI, CLAUDIA - Universita Di Padova
item BISCARINI, FILIPPO - National Research Council - Italy
item BARONE, VALERIA - Universita Di Catania
item BAGLIERI, ANDREA - Universita Di Catania
item SQUARTINI, ANDREA - Universita Di Padova
item CONCHERI, GUISEPPE - Universita Di Padova
item STEVANATO, PIERGIORGIO - Universita Di Padova

Submitted to: Euphytica
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 12/12/2017
Publication Date: 12/12/2017
Citation: Broccanello, C., McGrath, J.M., Panella, L.W., Richardson, K.L., Chiodi, C., Biscarini, F., Barone, V., Baglieri, A., Squartini, A., Concheri, G., Stevanato, P. 2017. A SNP mutation affects rhizomania-virus content of sugar beets grown on resistance-breaking soi. Euphytica. 214:14.

Interpretive Summary: Rhizomania or "crazy root" is the most devastating sugar beet disease worldwide, affecting every growing region and reducing average yields by nearly 70% in some areas. The only demonstratedly effective control for rhizomania has been genetic resistance, of which two genes have been shown to dramatically reduce yield loss and allow profitable sugar beet production. Breeding rhizomania resistance is difficult due to the need for bioassays and specialized production facilities, and genetic markers would be useful to allow more material to be screened for these and other rhizomania resistance genes. This work developed one such marker, SNP249, that correlates with sugar beet resistance to a recently identified rhizomania variant. This marker can be deployed to breed for resistance to this new rhizomania strain.

Technical Abstract: Rhizomania is one of the most devastating biotic stresses affecting sugar beet (Beta vulgaris L.). It is caused by Beet necrotic yellow vein virus (BNYVV) vectored by the plasmodiophorid Polymyxa betae K. The only means available to control the disease is the use of genetically resistant varieties. “Rizor” or “Holly” (Rz1) and WB42 (Rz2) have been the most widely used resistance sources in the commercial varieties. Recently, naturally occurring resistance-breaking (RB) rhizomania strains have been identified causing major concerns. The aim of this study was to identify SNP mutations that show associations with resistance to rhizomania in sugar beet plants grown under resistance-breaking (RB)-BNYVV soils. Rhizomania virus content was evaluated by indirect triple-antibody sandwich (TAS)-ELISA within two F2 segregating populations respectively grown on an AYPR and IV-BNYVV strain infected soils. Bulked segregant analysis (BSA) was performed. The resistant and susceptible plants were genotyped with a 384-SNPs panel. Of the 384 SNPs, SNP24 was found to associate with the resistance both to the AYPR strain (R2= 0.37; P=0.0004) and to the IV-BNYVV (R2= 0.09; P= 0.0074). Our results suggested that the SNP249 could be readily applicable for marker-assisted breeding of resistance to AYPR strain of rhizomania.