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ARS Home » Northeast Area » Beltsville, Maryland (BARC) » Beltsville Agricultural Research Center » Molecular Plant Pathology Laboratory » Research » Publications at this Location » Publication #344955

Research Project: Genome-Based Strategies and Physiological Biomarkers for Detection and Identification of plant Pathogenic Phytoplasmas and Spiroplasmas

Location: Molecular Plant Pathology Laboratory

Title: Relationship of bacterial growth and chemical events in the leaf apoplast during susceptible and resistant interactions

item Baker, Con
item MOCK, NORTON - Retired ARS Employee
item AVERYANOV, ANDREY - Collaborator

Submitted to: Free Radicals in Biology and Medicine
Publication Type: Abstract Only
Publication Acceptance Date: 11/14/2017
Publication Date: 11/20/2017
Citation: Baker, C.J., Mock, N.M., Averyanov, A.A. 2017. Relationship of bacterial growth and chemical events in the leaf apoplast during susceptible and resistant interactions. Free Radicals in Biology and Medicine.

Interpretive Summary:

Technical Abstract: The plant apoplast is one of the first battlegrounds for pathogens invading via leaf stomata. The apoplast is the border just outside the cell membrane and in a leaf, much of the apoplast is in direct contact or near the air space within the leaf. It is one of the most accessible parts of the plant for the study of early events in the bacteria/plant interactions and likewise it offers lucrative possibilities for plant disease control. Recent studies have shown that two simple phenolics, acetovanillone and acetosyringone, respond to bacterial infection within the first few hours after inoculation, but their timing and concentrations vary depending on the type of interaction: saprophytic, susceptible, or resistant. Here we describe a second phase of the interaction, about 10 h post inoculation, in which chlorogenic acids increase dramatically in pathogenic interactions, susceptible and resistant. Chlorogenic acid is stored in the plant vacuole and its appearance indicates leakage across the apoplast/membrane barrier, which in turn is likely to affect bacterial growth. Therefore we have tried to correlate bacterial multiplication with these chemical events in the three types of interactions. The oxidative environment of the apoplast during these early periods suggests that these phenolic compounds may influence the redox environment and directly inhibit bacterial multiplication during certain periods of the interaction.