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ARS Home » Southeast Area » Fort Pierce, Florida » U.S. Horticultural Research Laboratory » Citrus and Other Subtropical Products Research » Research » Publications at this Location » Publication #344757
Title: Bacterial and fungal next generation sequencing datasets and metadata from citrus infected with 'Candidatus liberibacter asiaticus'

Author
item GINNAN, NICHOLE - University Of California
item DANG, TYLER - University Of California
item BODAGHI, SOHRAB - University Of California
item RUEGGER, PAUL - University Of California
item PEACOCK, BETH - University Of California
item McCollum, Thomas
item VIDALAKIS, GEORGIOS - University Of California
item ROPER, CAROLINE - University Of California
item ROLSHAUSEN, PHILLIPE - University Of California
item BORNEMAN, JAMES - University Of California

Submitted to: Phytobiomes Journal
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 3/23/2018
Publication Date: 5/3/2018
Citation: Ginnan, N., Dang, T., Bodaghi, S., Ruegger, P.M., Peacock, B.B., McCollum, T.G., Vidalakis, G., Roper, C., Rolshausen, P., Borneman, J. 2018. Bacterial and fungal next generation sequencing datasets and metadata from citrus infected with 'Candidatus liberibacter asiaticus'. Phytobiomes Journal. https://doi.org/10.1094/PBIOMES-08-17-0032-A.
DOI: https://doi.org/10.1094/PBIOMES-08-17-0032-A

Interpretive Summary: Huanglongbing is a devastating disease of citrus. This disease has reduced citrus production in Florida by 70% since it was first discovered in Florida in 2005. It is becoming increasingly clear that microbes living on plant leaves and roots can be impacted by pathogens that cause disease. The entire population of microbes living on plant tissues is know as the phytobiome. The objective of the research reported on here was to characterize the microbiomes of citrus trees growing in orchards affected by Huanglongbing. The micirobiomes associated with citrus roots, leaves, and budwood, sampled from different varieties of citrus, grown in different regions of Florida were characterized by analysis of nucleotide sequences isolated from the various samples. Data on the diversity of fungi and bacteria growing on the trees provides baseline reference that will be used in subsequent research to determine if there are organisms in the citrus microbiome that are related to Huanglongbing disease development.

Technical Abstract: Plant-associated microbiomes are now intensively studied to determine if and how they influence plant health and disease. Citrus grown in the United States is currently being severely threatened by Huanglongbing (HLB) disease. We used next generation sequencing (NGS) of the rRNA ITS region to create a microbiome resource from citrus grown in an area of high HLB pressure, the state of Florida in the United States. We report the bacterial and fungal taxa associated with citrus roots, leaves and budwood sampled from the same trees. We also provide the metadata associated with these sequences, which include tissue type, citrus rootstock, citrus scion, geographical region and age of the trees.