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ARS Home » Northeast Area » Beltsville, Maryland (BARC) » Beltsville Agricultural Research Center » National Germplasm Resources Laboratory » Research » Publications at this Location » Publication #344401

Research Project: Characterizing and Detecting Pathogens to Ensure Safe Exchange of Plant Germplasm

Location: National Germplasm Resources Laboratory

Title: Complete genome sequence of Paris mosaic necrosis virus, a distinct member of the genus Potyvirus

Author
item Lan, Pingxiu - Yunnan Agricultural University
item Zhao, Jurun - Yunnan Agricultural University
item Zhou, Yalin - Yunnan Agricultural University
item Li, Yueyue - Yunnan Agricultural University
item Shen, Dingcai - Yunnan Agricultural University
item Liao, Qinchan - Yunnan Agricultural University
item Li, Ruhui
item Li, Fan - Yunnan Agricultural University

Submitted to: Archives of Virology
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 10/5/2017
Publication Date: 12/29/2017
Citation: Lan, P., Zhao, J., Zhou, Y., Li, Y., Shen, D., Liao, Q., Li, R., Li, F. 2017. Complete genome sequence of Paris mosaic necrosis virus, a distinct member of the genus Potyvirus. Archives of Virology. https://doi.org/10.1007/s00705-017-3649-x.

Interpretive Summary: Paris polyphylla var. yunnanensis (Dian Chonglou in Chinese) is an herb distributed primarily in southwest China that is used in traditional medicine. Overharvesting of wild populations and increasing demand by the pharmaceutical industry have spurred interest in producing it as a cultivated crop. However, this practice is causing an increased incidence of yield losses from diseases. In this study, a new virus was identified from plants with foliar mottle, mosaic and necrosis symptoms from fields in Yunnan province. The complete genomic sequence of the virus was determined. Analyses of the genetic information indicate it is a novel virus in the potyvirus group. A survey of cultivated herb fields in two counties of the province suggests the virus is common. This study provides the information necessary to classify the virus, and to develop detection methods necessary for disease management strategies.

Technical Abstract: The complete genomic sequence of a novel potyvirus was determined from Paris polyphylla var. yunnanensis. Its genomic RNA consists of 9,660 nucleotides (nt) excluding the 3’-terminal poly (A) tail, containing a single open reading frame (ORF) encoding a large polyprotein. The virus shares 52.1-69.7% nt sequence identities and 43.9-73.3% amino acid sequence identities with other members of the genus Potyvirus. Proteolytic cleavage sites and conserved motifs characterisitc of potyviruses were identified in the polyprotein and individual proteins. Phylogenetic analysis indicates the virus is most closely related to Wisteria vein mosaic virus in the BCMV subgroup. The results suggest the virus is a novel species in the genus Potyvirus, and which we tentatively name Paris mosaic necrosis virus.