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ARS Home » Plains Area » Stillwater, Oklahoma » Wheat, Peanut, and Other Field Crops Research » Research » Publications at this Location » Publication #344080

Research Project: Identification, Characterization, and Development of Insect-Resistant Wheat, Barley, and Sorghum Germplasm

Location: Wheat, Peanut, and Other Field Crops Research

Title: Characterization of Pm59, a novel powdery mildew resistance gene in Afghanistan wheat landrace PI 181356

Author
item Tan, Chengcheng
item LI, GENQIAO - Oklahoma State University
item Cowger, Christina
item CARVER, BRETT - Oklahoma State University
item Xu, Xiangyang

Submitted to: Theoretical and Applied Genetics
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 2/5/2018
Publication Date: 4/15/2018
Citation: Tan, C., Li, G., Cowger, C., Carver, B.F., Xu, X. 2018. Characterization of Pm59, a novel powdery mildew resistance gene in Afghanistan wheat landrace PI 181356. Theoretical and Applied Genetics. 131(5):1145-1152. https://doi.org/10.1007/s00122-018-3067-9.
DOI: https://doi.org/10.1007/s00122-018-3067-9

Interpretive Summary: Powdery mildew, caused by Blumeria graminis f. sp. tritici (Bgt), is an important foliar disease of wheat worldwide. In the Great Plains of the USA, Bgt isolates virulent to widely used powdery mildew resistance genes, such as Pm3a, were previously identified. The objectives of this study were to characterize the powdery mildew resistance gene in Afghanistan landrace PI 181356, which exhibited high resistance to Bgt isolates collected in southern Great Plains, and identify molecular markers for marker-assisted selection. Genetic analysis indicated that PI 181356 carries a single dominant gene, designated Pm59, in the terminal region of the long arm of chromosome 7A. Pm59 was mapped to an interval between sequence tag site (STS) markers Xmag1759 and Xmag1714. Pm59 is a novel powdery mildew resistance gene, and confers resistance to Bgt isolates collected from the Great Plains and the state of Montana. Therefore it can be used to breed powdery mildew-resistant cultivars in these regions. Xmag1759 is closely linked to Pm59 and can be used to select this gene in wheat breeding.

Technical Abstract: Powdery mildew, caused by Blumeria graminis f. sp. tritici (Bgt), is an important foliar disease of wheat worldwide. In the Great Plains of the USA, Bgt isolates virulent to widely used powdery mildew resistance genes, such as Pm3a, were previously identified. The objectives of this study were to characterize the powdery mildew resistance gene in Afghanistan landrace PI 181356, which exhibited high resistance to Bgt isolates collected in southern Great Plains, and identify molecular markers for marker-assisted selection. An F2 population and F2:3 lines derived from a cross between PI 181356 and OK105960-126135-3 were used in this study. Genetic analysis indicated that PI 181356 carries a single dominant gene, designated Pm59, in the terminal region of the long arm of chromosome 7A. Pm59 was mapped to an interval between sequence tag site (STS) markers Xmag1759 and Xmag1714 with genetic distances of 0.4 cM distal to Xmag1759 and 5.7 cM proximal to Xmag1714. Physical mapping suggested that Pm59 is in the distal bin 7AL 0.99-1.00. Pm59 is a novel powdery mildew resistance gene, and the estimated genetic distance between Pm59and Pm1, which also resides in 7AL 0.99-1.00, was 5.7 cM. Pm59 confers resistance to Bgt isolates collected from the Great Plains and the state of Montana, and can be used to breed powdery mildew-resistant cultivars in these regions. Xmag1759 is ideal for marker-assisted selection of Pm59 in wheat breeding.