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ARS Home » Northeast Area » Beltsville, Maryland (BARC) » Beltsville Agricultural Research Center » National Germplasm Resources Laboratory » Research » Publications at this Location » Publication #344017

Research Project: Characterizing and Detecting Pathogens to Ensure Safe Exchange of Plant Germplasm

Location: National Germplasm Resources Laboratory

Title: Molecular characterization of a novel Nucleorhabdovirus from black currant identified by high-throughput sequencing

Author
item Wu, Liping - Nanchang University
item Liu, Huawei - China Agricultural University
item Postman, Joseph
item Li, Ruhui

Submitted to: Archives of Virology
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 12/19/2017
Publication Date: 1/24/2018
Citation: Wu, L., Liu, H., Postman, J.D., Li, R. 2018. Molecular characterization of a novel Nucleorhabdovirus from black currant identified by high-throughput sequencing. Archives of Virology. https://doi.org/10.1007/s00705-018-3709-x.

Interpretive Summary: Black currant (Ribes nigrum) is a woody shrub that is widely cultivated for its nutrition-rich fruits. Several viruses and virus-like pathogens infect black currant, causing various diseases that reduce yields and market value of the fruit. In this study, a new virus was identified from a black currant accession imported from Europe by a sensitive sequencing technology. The complete genomic sequence of the virus was determined. Analyses of the genetic information show that it is a novel virus in the rhabdovirus group. The virus was also detected in one other currant accession from the USDA-ARS National Clonal Germplasm Repository in Oregon. The study provides the information necessary to classify the virus and to help develop detection methods for quarantine and certification programs.

Technical Abstract: Contigs with sequence similarities to several nucleorhabdoviruses were identified by high-throughput sequencing analysis from a black currant (Ribes nigrum L.) cultivar. The complete genomic sequence of this new nucleorhabdovirus is 14,432 nucleotides. Its genomic organization is typical of nucleorhabdovirus, containing six open reading frames in very similar arrangement. The virus, which is 41-52% nucleotide identical to other species of the genus Nucleorhabdovirus, is a new species we have provisionally named Black currant nucleorhabdovirus 1.