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ARS Home » Southeast Area » Griffin, Georgia » Plant Genetic Resources Conservation Unit » Research » Publications at this Location » Publication #343879

Title: Association analysis of cowpea mosaic virus (CPMV) resistance in the USDA cowpea germplasm collection

item BHATTARAI, GEHENDRA - University Of Arkansas
item SHI, AINONG - University Of Arkansas
item QIN, JUN - University Of Arkansas
item WENG, YUEJIN - University Of Arkansas
item Morris, John - Brad
item Pinnow, David
item BUCKLEY, BLAIR - Louisiana State University
item RAVELOMBOLA, WALTRAM - University Of Arkansas
item YANG, WEI - University Of Arkansas
item DONG, LINGDI - University Of Arkansas

Submitted to: Euphytica
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 8/28/2017
Publication Date: 9/7/2017
Citation: Bhattarai, G., Shi, A., Qin, J., Weng, Y., Morris, J.B., Pinnow, D.L., Buckley, B., Ravelombola, W., Yang, W., Dong, L. 2017. Association analysis of cowpea mosaic virus (CPMV) resistance in the USDA cowpea germplasm collection. Euphytica. 213:230-242.

Interpretive Summary: Cowpea is an important crop grown worldwide for use as a fresh vegetable, dry grain, and animal feed. Cowpea mosaic virus causes severe losses in cowpea yields and genetic resistance is the most effective control method. The association analysis was conducted for cowpea mosaic virus resistance in 333 cowpea samples from the USDA, ARS, PGRCU cowpea collection representing 39 countries. DNA sequencing was used to identify differences in single nucleotides among the cowpea samples. A total of 1,033 single nucleotide markers were identified. Using molecular genetic techniques and statistical analysis, six single nucleotide markers were found to be strongly associated with cowpea mosaic virus resistance. These genetic markers can be used by breeders to develop cowpea mosaic virus resistant varieties.

Technical Abstract: Cowpea [Vigna unguiculata (L.) Walp.] is an important legume crop, widely grown in Africa, South America, South Asia, Southeast Asia, and the southern United States. Cowpea is consumed as both fresh vegetable and dry grain, and as an animal feed and fodder, and it is a major dietary protein source that complements cereal-based diet. Cowpea mosaic virus (CPMV) causes a severe yield loss of cowpea in many areas worldwide notably in the Africa. Utilization of host genetic resistance is the most effective control method for the viral disease. The objective of this research is to conduct genome-wide association analysis (GWAS) and identify single nucleotide polymorphism (SNP) markers associated with CPMV resistance in cowpea. Three hundred and thirty-three cowpea germplasm accessions, originally collected from 39 different countries and 1033 SNPs identified from genotyping by sequencing (GBS) approach were used in this study. Single marker regression (SMR), general linear model (GLM), and mixed linear model (MLM) in Tassel 5 were used for association analysis of CPMV resistance. Six SNP markers (C35069548_1883, scaffold65342_6794, scaffold66293_6549, scaffold95805_2175, C35081948_540, and scaffold17319_4417) were strongly associated with the CPMV resistance, of which the first three were associated for immune and the remaining three were associated with hypersensitive response. SNP markers identified in this research will be a potential tool to use in cowpea molecular breeding to develop CPMV resistant cultivars through marker-assisted selection (MAS).