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ARS Home » Midwest Area » Ames, Iowa » Corn Insects and Crop Genetics Research » Research » Publications at this Location » Publication #343685

Research Project: Disease Resistance Signaling in Cereal Crops

Location: Corn Insects and Crop Genetics Research

Title: A robust and efficient method for the extraction of plant extracellular surface lipids as applied to the analysis of silks and seedling leaves of maize

Author
item Loneman, Derek - Iowa State University
item Peddicord, Layton - Iowa State University
item Al-rashid, Amani - Iowa State University
item Nikolau, Basil - Iowa State University
item Lauter, Nicholas
item Yandeau-nelson, Marna - Iowa State University

Submitted to: PLoS One
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 6/6/2017
Publication Date: 7/11/2017
Citation: Loneman, D.M., Peddicord, L., Al-Rashid, A., Nikolau, B.J., Lauter, N.C., Yandeau-Nelson, M.D. 2017. A robust and efficient method for the extraction of plant extracellular surface lipids as applied to the analysis of silks and seedling leaves of maize. PLoS One. 12(7):e0180850. doi:10.1371/journal.pone.0180850.

Interpretive Summary: Understanding how crop plants protect themselves against pathogens, herbivores, and weather is critical for engineering crop improvement. The plant cuticle is the outermost chemical barrier between aerial portions of a plant and the external environment, and therefore essential for stress tolerance. The cuticle is a structure deposited by epidermal cells and consists of an insoluble polyester, cutin, and a complex chemical mixture of extracellular lipids that are derived from fatty acids biosynthesized by the plant. This research developed new extraction and sample handling methods for biochemically characterizing the array of hundreds of surface lipid metabolites present on plant cuticles. The value of the new methods was demonstrated using corn silks and corn leaves, showing dramatic variation in the concentration and composition of the extracellular surface lipid metabolomes for these tissues across several types of corn and in response to developmental and environmental cues. These methods are now available for use in large-scale studies focused on optimizing the surface lipid metabolome for crop protection.

Technical Abstract: Aerial plant organs possess a diverse array of extracellular surface lipids, including both non-polar and amphipathic constituents that collectively provide a primary line of defense against environmental stressors. Extracellular surface lipids on the stigmatic silks of maize are composed primarily of saturated and unsaturated linear hydrocarbons, as well as fatty acids, and aldehydes. To efficiently extract lipids of differing polarities from maize silks, five solvent systems (hexanes; hexanes:diethyl ether (95:5); hexanes:diethyl ether (90:10); chloroform:hexanes (1:1) and chloroform) were tested by immersing fresh silks in solvent for different extraction times. Surface lipid recovery and the relative composition of individual constituents were impacted to varying degrees depending on solvent choice and duration of extraction. Analyses were performed using both silks and leaves to demonstrate the utility of the solvent- and time-optimized protocol in comparison to extraction with the commonly used chloroform solvent. Overall, the preferred solvent system was identified as hexanes:diethyl ether (90:10), based on its effectiveness in extracting surface hydrocarbons and fatty acids as well as its reduced propensity to extract presumed internal fatty acids. Metabolite profiling of wildtype and glossy1 seedlings, which are impaired in surface lipid biosynthesis, demonstrated the ability of the preferred solvent to extract extracellular surface lipids rich in amphipathic compounds (aldehydes and alcohols). In addition to the expected deficiencies in dotriacontanal and dotriacontan-1-ol for gl1 seedlings, an unexpected increase in fatty acid recovery was observed in gl1 seedlings extracted in chloroform, suggesting that chloroform extracts lipids from internal tissues of gl1 seedlings. This highlights the importance of extraction method when evaluating mutants that have altered cuticular lipid compositions. Finally, metabolite profiling of silks from maize inbreds B73 and Mo17, exposed to different environments and harvested at different ages, revealed differences in hydrocarbon and fatty acid composition, demonstrating the dynamic nature of surface lipid accumulation on silks.