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ARS Home » Northeast Area » Ithaca, New York » Robert W. Holley Center for Agriculture & Health » Emerging Pests and Pathogens Research » Research » Publications at this Location » Publication #343235

Research Project: Emerging and Invasive Nematode and Virus Pathogens Affecting Potato

Location: Emerging Pests and Pathogens Research

Title: Mapping loci that control tuber and foliar symptoms caused by PVY in Autotetraploid Potato (Solanum tuberosum L.)

item DA SILVA, WASHINGTON - Cornell University - New York
item Ingram, Jason
item HACKETT, CHRISTINE - The James Hutton Institute
item COOMBS, JOSEPH - Michigan State University
item DOUCHES, DAVID - Michigan State University
item BRYAN, GLENN - The James Hutton Institute
item DEJONG, WALTER - Cornell University - New York
item Gray, Stewart

Submitted to: G3, Genes/Genomes/Genetics
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 9/1/2017
Publication Date: 9/13/2017
Citation: Da Silva, W., Ingram, J.T., Hackett, C., Coombs, J., Douches, D., Bryan, G., Dejong, W., Gray, S.M. 2017. Mapping loci that control tuber and foliar symptoms caused by PVY in Autotetraploid Potato (Solanum tuberosum L.). G3, Genes/Genomes/Genetics. doi: 10.1534/g3.117.300264.

Interpretive Summary: Emerging strains of Potato virus Y are more damaging to the U.S. potato industry because they can cause a tuber necrotic disease in some potato cultivars which reduces the quality of the potato and renders it unmarketable. Currently there is no method to determine if a potato cultivar is susceptible to tuber necrotic disease other than infecting the potato plant and observing the potatoes generated from that plant. This takes several months and breeders would like an easy and quick way to evaluate breeding material so they can eliminate susceptible material early on. This research is the first step in developing genetic markers that breeders can use to screen breeding material for its susceptibility to tuber necrosis. A region on one potato chromosome was found to be linked to tuber necrosis symptoms. Using this information researchers can now begin the process of identifying the genes in that chromosome region that are responsible for tuber necrosis symptoms.

Technical Abstract: Potato tuber necrotic ringspot disease (PTNRD) is a tuber deformity associated with infection by the tuber necrotic strain of Potato virus Y (PVYNTN). PTNRD negatively impacts tuber quality and marketability and poses a serious threat to seed and commercial potato production worldwide. PVYNTN symptoms differ in the cultivars Waneta and Pike: Waneta expresses severe PTNRD and foliar mosaic with vein and leaf necrosis, whereas Pike does not express PTNRD and mosaic is the only foliar symptom. To map loci that influence tuber and foliar symptoms, 236 F1 progeny of a cross between Waneta and Pike were inoculated with PVYNTN isolate NY090029 and genotyped using 12,808 Potato SNPs. Foliar symptom type and severity were monitored for 10 weeks, while tubers were evaluated for PTNRD expression at harvest and again after 60 days in storage. Pairwise correlation analyses indicate a strong association between PTNRD and vein necrosis (Tau = 0.4195). QTL analyses revealed major-effect QTLs on chromosomes 4 and 5 for mosaic, 4 for PTNRD, and 5 for foliar-necrosis symptoms. Locating QTLs associated with PVY-related symptoms provides a foundation for breeders to develop markers that can be used to screen out potato clones with undesirable phenotypes, e.g., those likely to develop PTNRD or to be symptomless carriers of PVY.