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ARS Home » Southeast Area » Stoneville, Mississippi » Warmwater Aquaculture Research Unit » Research » Publications at this Location » Publication #342820

Research Project: Biophotonics – Technology Development in Animal Research and Production Systems

Location: Warmwater Aquaculture Research Unit

Title: Equine ovarian tissue viability after cryopreservation and in vitro culture

item Gastal, Gustavo - Southern Illinois University
item Alves, Benner - Universidade Estadual Do Ceara
item Aguiar, Nascimento - Southern Illinois University
item Amaral, K - Universidade Estadual Do Ceara
item De, Tarso - Universidade Estadual Do Ceara
item Ghassan, Ishak - Southern Illinois University
item Cavinder, Clay - Mississippi State University
item Feugang, Jean - Mississippi State University
item Gastal, Eduardo - Southern Illinois University

Submitted to: Theriogenology
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 4/21/2017
Publication Date: N/A
Citation: N/A

Interpretive Summary: Ovarian preservation is another way to restore fertility of females undergoing critical conditions or death. However, the production of mature viable gametes after in vitro culture or transplantation of ovarian grafts are still challenging. Here evaluated the survivability of mare ovaries after cryopreservation. Results indicated that successful ovarian cryopreservation is possible and is dependable of employed protocols. Findings will advance knowledge toward the development of adequate cryopreservation protocols for long-term banking of female gametes.

Technical Abstract: The efficiency of several cryoprotective agents were compared using both slow-freezing and vitrification methods. Results indicate that the viability of ovarian tissue cells increases when DMSO (slow-freezing) and ethylene glycol (vitrification) are used.