Location: Warmwater Aquaculture Research UnitTitle: Equine ovarian tissue viability after cryopreservation and in vitro culture
|Gastal, Gustavo - Southern Illinois University|
|Alves, Benner - Universidade Estadual Do Ceara|
|Aguiar, Nascimento - Southern Illinois University|
|Amaral, K - Universidade Estadual Do Ceara|
|De, Tarso - Universidade Estadual Do Ceara|
|Ghassan, Ishak - Southern Illinois University|
|Cavinder, Clay - Mississippi State University|
|Feugang, Jean - Mississippi State University|
|Gastal, Eduardo - Southern Illinois University|
Submitted to: Theriogenology
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 4/21/2017
Publication Date: N/A
Interpretive Summary: Ovarian preservation is another way to restore fertility of females undergoing critical conditions or death. However, the production of mature viable gametes after in vitro culture or transplantation of ovarian grafts are still challenging. Here evaluated the survivability of mare ovaries after cryopreservation. Results indicated that successful ovarian cryopreservation is possible and is dependable of employed protocols. Findings will advance knowledge toward the development of adequate cryopreservation protocols for long-term banking of female gametes.
Technical Abstract: The efficiency of several cryoprotective agents were compared using both slow-freezing and vitrification methods. Results indicate that the viability of ovarian tissue cells increases when DMSO (slow-freezing) and ethylene glycol (vitrification) are used.