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ARS Home » Southeast Area » Stoneville, Mississippi » Warmwater Aquaculture Research Unit » Research » Publications at this Location » Publication #342819

Research Project: Biophotonics – Technology Development in Animal Research and Production Systems

Location: Warmwater Aquaculture Research Unit

Title: A systems biology approach using transcriptomic data reveals genes and pathways in porcine skeletal muscle affected by dietary lysine

Author
item Wang, Taiji - Mississippi State University
item Feugang, Jean - Mississippi State University
item Crenshaw, Mark - Mississippi State University
item Regmi, N - Mississippi State University
item Blanton Jr, John - Mississippi State University
item Liao, Shengfa - Mississippi State University

Submitted to: International Journal of Molecular Sciences
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 4/18/2017
Publication Date: 4/21/2017
Citation: Wang, T., Feugang, J.M., Crenshaw, M.A., Regmi, N., Blanton Jr, J.R., Liao, S.F. 2017. A systems biology approach using transcriptomic data reveals genes and pathways in porcine skeletal muscle affected by dietary lysine. International Journal of Molecular Sciences. 18:885.

Interpretive Summary: The growth and development of skeletal muscle of pigs requires dietary supply of proteins, meaning amino acids. Pigs are unable to synthesize lysine amino acid, making their dietary inclusion essential for pig growth performance and meat carcass characteristics. The molecular mechanisms through which lysine exerts its effects remain unclear. Here we evaluated the effect of deficient, adequate, and excess lysine diets on gene expression profiles of skeletal muscles (longissimus dorsi) of finishing pigs. Results revealed 674 genes that were significantly differentially expressed (DEG) between all treatments. The study narrowed the focus on 131 highly DEG of which only 60 were fully annotated in the microarray. Hence, only 13 DEG were shared between all treatments, leaving numerous down- and up-regulated DEG in pairwise diet comparisons. These expression changes were associated with several biological pathways, especially those related to the regulation of protein turnover and lipid metabolism affecting muscle growth.

Technical Abstract: Meeting the increasing market demands for pork products requires improvement of the feed efficiency of growing pigs. The use of Affymetrix Porcine Gene 1.0 ST array containing 19,211 genes in this study provides a comprehensive gene expression profile of skeletal muscle of finishing pigs in response to various dietary lysine levels.