|KWON, JUNG-HOON - Konkuk University|
|LEE, DONG-HUN - Orise Fellow|
|NOH, JIN-YONG - Konkuk University|
|YUK, SEONG-SU - Konkuk University|
|JEONG, SOL - Konkuk University|
|LEE, S - Konkuk University|
|WOO, C - Environmental Research Complex|
|SHIN, J - Environmental Research Complex|
|SONG, CHANG-SEON - Konkuk University|
Submitted to: Transboundary and Emerging Diseases
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 3/6/2018
Publication Date: 5/4/2018
Citation: Kwon, J., Lee, D., Swayne, D.E., Noh, J., Yuk, S., Jeong, S., Lee, S.H., Woo, C., Shin, J.H., Song, C. 2018. Experimental infection of H5N1 and H5N8 highly pathogenic avian influenza viruses in Northern Pintail (Anas acuta). Transboundary and Emerging Diseases. 65(5):1367-1371. https://doi.org/10.1111/tbed.12872.
Interpretive Summary: The deadly H5 highly pathogenic avian influenza (HPAI) (group 126.96.36.199) viruses that have spread by wild birds is of great concern. In December 2014, it was introduced to North America with eradication in June 2015. Long-distance migrating ducks, such as Northern Pintail (Anas Acuta), were strongly suspected of being a source of Asia to North American transmission. In our study, we demonstrated that H5 HPAI virus infected Northern Pintails with either H5N1 (group 188.8.131.52) or H5N8 (group 184.108.40.206) virus were not ill and did not die, but the H5N8 (group 220.127.116.11) virus was better at growing in the birds transmitting to other birds. This study highlights the role migratory ducks can have in spreading some H5 HPAI viruses.
Technical Abstract: The wide geographic spread of Eurasian Goose/Guangdong lineage highly pathogenic avian influenza (HPAI) clade 18.104.22.168 viruses by wild birds is of great concern. In December 2014, an H5N8 HPAI clade 22.214.171.124A virus was introduced to North America. Long-distance migratory wild aquatic birds between East Asia and North America, such as Northern Pintail (Anas Acuta), were strongly suspected of being a source of intercontinental transmission. In this study, we evaluated the pathogenicity, infectivity and transmissibility of an H5N8 HPAI clade 126.96.36.199A virus in Northern Pintails and compared the results to that of an H5N1 HPAI clade 188.8.131.52 virus. All of Northern Pintails infected with either H5N1 or H5N8 virus lacked clinical signs and mortality, but the H5N8 clade 184.108.40.206 virus was more efficient at replicating within and transmitting between Northern Pintails than the H5N1 clade 220.127.116.11 virus. The H5N8 infected birds shed high titer of viruses from oropharynx and cloaca, which in the field supported virus transmission and spread. This study highlights the role of wild waterfowl in the intercontinental spread of some HPAI viruses. Migratory aquatic birds should be carefully monitored for the early detection of H5 clade 18.104.22.168 and other HPAI viruses.