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ARS Home » Southeast Area » Raleigh, North Carolina » Soybean and Nitrogen Fixation Research » Research » Publications at this Location » Publication #342777

Research Project: Increasing the Competitiveness of U.S. Soybeans in Global Markets through Genetic Diversity, Genomics, and Plant Breeding

Location: Soybean and Nitrogen Fixation Research

Title: Mapping and confirmation of loci for salt tolerance in a novel germplasm soybean cultivar Fiskeby III

Author
item DO, TUYEN - University Of Missouri
item VUONG, TRI - University Of Missouri
item DUNN, DAVID - University Of Missouri
item SMOTHERS, SCOTTY - University Of Missouri
item PATIL, GUNVANT - University Of Missouri
item YUNGBLUTH, DENNIS - University Of Missouri
item CHEN, PENGYIN - University Of Missouri
item SCABOO, ANDREW - University Of Missouri
item XU, DONG - University Of Missouri
item Carter Jr, Thomas
item SHANNON, J. - University Of Missouri
item NGUYEN, HENRY - University Of Missouri

Submitted to: Journal of Theoretical and Applied Genetics
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 11/4/2017
Publication Date: 11/18/2017
Citation: Do, T.D., Vuong, T.D., Dunn, D., Smothers, S., Patil, G., Yungbluth, D.C., Chen, P., Scaboo, A., Xu, D., Carter Jr, T.E., Shannon, J.G., Nguyen, H.T. 2017. Mapping and confirmation of loci for salt tolerance in a novel germplasm soybean cultivar Fiskeby III. Journal of Theoretical and Applied Genetics. 131:513-524. https://doi.org/10.1007/s00122-017-3015-0.
DOI: https://doi.org/10.1007/s00122-017-3015-0

Interpretive Summary: High salt concentration is an important barrier to crop production in many areas of the world. In the USA, salt build up is a common problem in the Delta region of the South and in eastern counties of North Carolina, Virginia, and Maryland. Soybean is commonly grown in these areas and suffers damage on hundreds of thousands of acres in most years. For these reasons, soybean breeders are actively developing new salt tolerant soybean varieties for farm use. The paper reports on a new source of salt tolerance in soybean (a Swedish variety called Fiskeby III), its inheritance, and the genomic location of the its salt tolerance genes. This new information will help soybean breeders be more efficient in their development of new salt tolerant varieties.

Technical Abstract: Breeding soybean for tolerance to high salt conditions is important in some regions of the USA and world. Soybean cultivar Fiskeby III (PI 438471) in maturity group 000 has been reported to be highly tolerant to multiple abiotic stress conditions, including salinity. In this study, a mapping population of 132 F2 families derived from a cross of cultivar Williams 82 (PI 518671, moderately salt sensitive) and Fiskeby III (salt tolerant) were analyzed to map salt tolerance genes. The evaluation for salt tolerance was performed by analyzing leaf scorch score (LSS), chlorophyll content ratio (CCR), leaf sodium content (LSC), and leaf chloride content (LCC) after treatment with 120 mM NaCl under greenhouse conditions. Genotypic data for the F2 population was obtained using the SoySNP6K Illumina Infinium BeadChip assays. A major allele from Fiskeby III significantly associated with LSS, CCR, LSC, and LCC on chromosome (Chr.) 03 with LOD scores of 19.1, 11.0, 7.7, and 25.6, respectively. In addition, a second locus associated with salt tolerance for LSC was detected and mapped on Chr. 13 with a LOD score of 4.6 and an R2 of 11.5%. Three gene-based molecular markers (Salt-20, Salt14056 and Salt11655) on Chr. 03 showed a strong predictive association with phenotypic salt tolerance in the present mapping population. These molecular markers will be useful for marker-assisted selection to improve salt tolerance in soybean.