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ARS Home » Southeast Area » Dawson, Georgia » National Peanut Research Laboratory » Research » Publications at this Location » Publication #342746

Research Project: Developing Strategies to Identify Useful Genes in Peanut and Breeding High Yielding Peanut Varieties and Germplasm

Location: National Peanut Research Laboratory

Title: Mutagenesis of FAD2 genes in peanut with CRISPR/Cas9

item YUAN, MEI - Tuskegee University
item ZHU, JUN - Tuskegee University
item LEE, CRYSTAL - Tuskegee University
item PRAKASH, C - Tuskegee University
item HE, GUOHAO - Tuskegee University
item Dang, Phat
item CHEN, CHARLES - Auburn University

Submitted to: Meeting Abstract
Publication Type: Abstract Only
Publication Acceptance Date: 5/1/2017
Publication Date: N/A
Citation: N/A

Interpretive Summary:

Technical Abstract: The CRISPR/Cas9 system is known for its precise and efficient gene-editing of a targeted region in a variety of organisms including plants. We targeted FAD2 gene region to perform CRISPR/Cas9 gene-editing in peanut. The FAD2 gene encodes fatty acid desaturase which catalyzes the conversion of oleic acid to linoleic acid. Modification or disruption of the FAD2 gene can inactivate this gene and reduce the enzymatic activity of the desaturase to accumulate oleic acid in seeds, thus, increase the ratio of O/L, as oleic acid has much health benefits. In this study, we not only identified the same mutations as previously reported in the natural mutant line ‘F435’ with a high level of oleate, but also generated a new mutation. The new mutation would provide an additional source for high level of oleate. Our preliminary results thus suggest that CRISPR/Cas9 gene-editing system can be a useful tool to generate mutant lines with varying genetic backgrounds to breed for high oleic content in peanut.