Location: National Peanut Research LaboratoryTitle: Domestication of perennial fruit trees: the case of mamey (Pouteria sapota) in Mexico
|MARTINEZ-CASTILLO, JAMIE - Yucatan Center For Scientific Research|
|Arias De Ares, Renee|
Submitted to: Meeting Abstract
Publication Type: Abstract Only
Publication Acceptance Date: 8/9/2016
Publication Date: N/A
Technical Abstract: The tropical plant Pouteria sapota (Jacq.) is known for its edible fruits that contain unique carotenoids, and for the chemicals extracted from its bark, leaves and roots having fungitoxic, insecticidal, anti-inflamatory, anti-oxidant and tyrosinase inhibitory activities. Currently, there is no genetic information about this species - only one entry of 443bp in GenBank. We did high throughput sequencing of microsatellite-enriched libraries of P. sapota, submitted 5223 DNA contig sequences with a total of 1.8 Mbp to GenBank, developed 384 microsatellites markers and tested the latter on 31 P. sapota samples from 11 populations (7 collected from the wild, 4 cultivated) from Mexico and Puerto Rico. BLAST analysis to gene ontology of the DNA sequences containing microsatellites that effectively discriminated these populations, showed association to physiological activities, e.g., folate synthesis, phototropism, defense against insects, phytoalexin synthesis, seed maturation, pathogen defense and hormone signaling. In samples collected from the wild, microsatellites at 62 loci detected one or more alleles that were not observed among the cultivated populations tested. Potential applications of the developed microsatellite markers screening germplasm collections, for ancestry and domestication studies are discussed.