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ARS Home » Southeast Area » Gainesville, Florida » Center for Medical, Agricultural and Veterinary Entomology » Mosquito and Fly Research » Research » Publications at this Location » Publication #342267

Research Project: Biting Arthropod Surveillance and Control

Location: Mosquito and Fly Research

Title: Rhanterium epapposum Oliv. essential oil: Chemical composition and antimicrobial, insect-repellent and anticholinesterase activities

item DEMIRCI, BETUL - Anadolu Universtiy
item YUSUFOGLU, HASAN - Sattam Bin Abdulaziz University
item TABANCA, NURHAYAT - University Of Florida
item TEMEL, HALIDE - Anadolu Universtiy
item Bernier, Ulrich
item AGRAMONTE, NATASHA - University Of Florida
item ALQASOUMI, SALEH - King Saud University
item AL-REHAILY, ADNAN - King Saud University
item CAN BASER, KEMAL - Anadolu Universtiy
item DEMIRCI, FATIH - Anadolu Universtiy

Submitted to: Saudi Pharmaceutical Journal
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 10/14/2016
Publication Date: 7/25/2017
Citation: Demirci, B., Yusufoglu, H.S., Tabanca, N., Temel, H.E., Bernier, U.R., Agramonte, N.M., Alqasoumi, S.I., Al-Rehaily, A.J., Can Baser, K.H., Demirci, F. 2017. Rhanterium epapposum Oliv. essential oil: Chemical composition and antimicrobial, insect-repellent and anticholinesterase activities. Saudi Pharmaceutical Journal. 25(5):703-708.

Interpretive Summary: The essential oil (EO) of the aerial parts of Rhanterium epapposum Oliv. (Asteraceae), was obtained by hydrodistillation. The oil was subsequently analyzed by both GC-FID and GCMS, simultaneously. Forty-five components representing 99.2% of the oil composition were identified. The most abundant compounds were camphene (38.5%), myrcene (17.5%), limonene (10.1%) and a-pinene (8.7%). Referring to the ethnobotanical utilization, an insecticidal assay was performed, where the oil repelled the yellow fever mosquito Aedes aegypti L. at a minimum effective dose (MED of 0.035 ± 0.010 mg/cm2) compared to the positive control DEET (MED of 0.015± 0.004 mg/cm2). Additionally, the in vitro antimicrobial activity against a panel of pathogens was determined using a microdilution method. The acetyl- and butyrylcholine esterase inhibitory activities were measured using the colorimetric Ellman method. The bioassay results showed that the oil was rather moderate in antimicrobial and cholinesterase inhibitions when compared to the standard compounds.

Technical Abstract: Essential oils from Rhanterium epapposum Oliv. (Asteraceae) was investigated for its repellent, antimicrobial and acetyl- and butyrylcholine esterase inhibitory activities. The oil showed good repellent activity while oils demonstrated weak in antimicrobial and cholinesterase inhibitions. Terpenoids constituted the majority of the oil (93.8%), of which monoterpenoids (91.58%) were the most predominant.