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ARS Home » Plains Area » Manhattan, Kansas » Center for Grain and Animal Health Research » Hard Winter Wheat Genetics Research » Research » Publications at this Location » Publication #341948

Research Project: Genetic Improvement of Hard Winter Wheat to Biotic and Abiotic Stresses

Location: Hard Winter Wheat Genetics Research

Title: Genotyping-by-sequencing derived high-density linkage map and its Aapplication to QTL mapping of flag leaf traits in bread wheat

item HUSSAIN, WASEEM - University Of Nebraska
item BAENZIGER, PETER - University Of Nebraska
item BELAMKAR, VIKAS - University Of Nebraska
item Guttieri, Mary
item VENEGAS, JORGE - University Of Nebraska
item EASTERLY, AMANDA - University Of Nebraska
item POLAND, JESSE - Kansas State University

Submitted to: Nature Scientific Reports
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 11/6/2017
Publication Date: 11/27/2017
Citation: Hussain, W., Baenziger, P., Belamkar, V., Guttieri, M.J., Venegas, J., Easterly, A., Poland, J. 2017. Genotyping-by-sequencing derived high-density linkage map and its Aapplication to QTL mapping of flag leaf traits in bread wheat. Nature Scientific Reports. Scientific Reports 7, Article number: 16394 (2017). doi: 10.1038/s41598-017-16006-z.

Interpretive Summary: This report describes the use of next-generation sequencing information to develop a genetic map of a hard winter wheat population that segregates for many important traits for wheat producers in the Great Plains of the United States. The validity of the map was confirmed using two wheat characteristics with known genetic locations, chaff color and leaf surface wax. The map then was used to identify genomic regions that affect the geometry of the wheat flag leaf, which is the terminal leaf and the most important light-harvesting leaf for wheat grain yield. Two regions of the genome with significant effects of leaf length and width were identified. Genetic markers in these regions may be useful in breeding for improved wheat yield.

Technical Abstract: Hard red winter wheat parents ‘Harry’ (drought tolerant) and ‘Wesley’ (drought susceptible) was used to develop a recombinant inbred population to identify genomic regions associated with drought and adaptation. To precisely map genomic regions high-density linkage maps are a prerequisite. In this study genotyping-by- sequencing (GBS) was used to construct the high-density linkage map. The map contained 3,641 markers distributed on 21 chromosomes and spanned 1,959 cM with an average distance of 1.79 cM between adjacent markers. The constructed linkage map revealed strong collinearity in marker order across 21 chromosomes with POPSEQ-v2.0, which was based on a high-density linkage map. The accuracy of the map for QTL mapping was demonstrated by co-localizing the genes to previously mapped genomic regions for two highly heritable traits, chaff color and leaf cuticular wax. Applicability of linkage map for QTL mapping of three quantitative traits, flag leaf length, width, and area, identified 21 QTLs in four environments, and QTL expression varied across the environments. Two major stable QTL, one each for flag leaf length (Qfll.hww-7A) and flag leaf width (Qflw.hww-5A) were identified. The map constructed will facilitate QTL and fine mapping of quantitative traits, map-based cloning, comparative mapping, and in marker-assisted wheat breeding endeavors.