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ARS Home » Northeast Area » Beltsville, Maryland (BHNRC) » Beltsville Human Nutrition Research Center » Diet, Genomics and Immunology Laboratory » Research » Publications at this Location » Publication #341753
Title: Inhibitory effect of piceatannol on TNF-a mediated inflammation and insulin resistance in 3T3-L1 adipocytes

Author
item LI, YANGANG - Beijing Advanced Innovation Center For Food Nutrition And Human Health, Beijing Technology & Busine
item YANG, PUYU - Xan Jiao Tong University
item CHANG, QIMENG - Fudan University
item WANG, JING - Beijing Advanced Innovation Center For Food Nutrition And Human Health, Beijing Technology & Busine
item LIU, JIE - Beijing Advanced Innovation Center For Food Nutrition And Human Health, Beijing Technology & Busine
item YUAN, LV - Xan Jiao Tong University
item Wang, Thomas - Tom
item GAO, BOYAN - University Of Maryland
item YU, LIANGLI (LUCY) - University Of Maryland

Submitted to: Journal of Agricultural and Food Chemistry
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 5/23/2017
Publication Date: 5/23/2017
Citation: Li, Y., Yang, P., Chang, Q., Wang, J., Liu, J., Yuan, L., Wang, T.T., Gao, B., Yu, L. 2017. Inhibitory effect of piceatannol on TNF-a mediated inflammation and insulin resistance in 3T3-L1 adipocytes. Journal of Agricultural and Food Chemistry. 65(23). https://doi.org/10.1021/acs.jafc.7b01567.
DOI: https://doi.org/10.1021/acs.jafc.7b01567

Interpretive Summary: Consumption of fruits and vegetables are associated with better health. However, the molecular mechanisms, as well as active components underlying their health-promoting effects are less clear. Piceatannol, a bioactive component in fruits such as grapes and blueberry, was examined for its potential in decreasing the inflammatory activities in the co-cultured adipocytesand macrophages system and suppressing TNF-a mediated inflammation and the related insulin resistance using a 3T3-L1 adipocytes model. Piceatannol treatment significantly reduced the release of inflammatory cytokines of TNF-a and MCP-1 in the co-cultured system and inhibited TNF-a induced expression of IL-6 and MCP-1 both at mRNA and protein levels in the 3T3-L1 adipocytes. Piceatannol also partially improved the malfunction of insulin- stimulated glucose uptake, which was reduced by TNF-a in 3T3-L1 adipocytes. Furthermore, the inhibitions were mediated by the significant blocking of I kappa B alpha phosphorylation and NF-kappa B activation through suppressing nuclear translocation of NF-kappa B p65 along with JNK-MAPK activation. In addition, Akt-dependent FoxO1 signaling pathway was involved in the restoration of insulin-stimulated glucose uptake through suppressing the down-regulation of phosphorylation of Akt and FoxO1 expressions. The information may be used by basic, as well as translation scientists to further elucidate the beneficial effects of consuming fruits and vegetables.

Technical Abstract: Piceatannol, a bioactive component in fruits such as grapes and blueberry, was examined for its potential in decreasing the inflammatory activities in the co-cultured adipocytes and macrophages system and suppressing TNF-a mediated inflammation and the related insulin resistance using a 3T3-L1 adipocytes model. Piceatannol treatment significantly reduced the release of inflammatory cytokines of TNF-a and MCP-1 in the co-cultured system and inhibited TNF-a induced expression of IL-6 and MCP-1 both at mRNA and protein levels in the 3T3-L1 adipocytes. Piceatannol also partially improved the malfunction of insulin stimulated glucose uptake, which was reduced by TNF-ain 3T3-L1 adipocytes. Furthermore, the inhibitions were mediated by the significant blocking of I kappa B phosphorylation and NF-kappa B activation through suppressing nuclear translocation of NF-kappa B p65 along with JNK-MAPK activation. In addition, Akt-dependent FoxO1 signaling pathway was involved in the restoration of insulin-stimulated glucose uptake through suppressing the down-regulation of phosphorylation of Akt and FoxO1 expressions.