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ARS Home » Pacific West Area » Hilo, Hawaii » Daniel K. Inouye U.S. Pacific Basin Agricultural Research Center » Tropical Plant Genetic Resources and Disease Research » Research » Publications at this Location » Publication #341401

Research Project: Molecular Resources for the Improvement of Tropical Ornamental and Fruit Crops

Location: Tropical Plant Genetic Resources and Disease Research

Title: Cytotoxicity and induction of the apoptotic activity of hirsutinolide series/sesquiterpene lactones from Vernonia cinerea on human colorectal cancer cells (COLO 205)

Author
item JONGRUNGRUANGCHOK, SUCHADA - Rangsit University
item PRADUBYAT, NALINEE - Rangsit University
item SONGSAK, THANAPAT - Rangsit University
item JARINTANUM, F - Rangsit University
item Wall, Marisa
item CHANG, LENG CHEE - University Of Hawaii
item WONGWIWATTHANANUKIT, SUPAKIT - University Of Hawaii

Submitted to: Journal of Current Science and Technology
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 5/22/2019
Publication Date: 6/18/2019
Citation: Jongrungruangchok, S., Pradubyat, N., Songsak, T., Jarintanum, F., Wall, M.M., Chang, L., Wongwiwatthananukit, S. 2019. Cytotoxicity and induction of the apoptotic activity of hirsutinolide series/sesquiterpene lactones from Vernonia cinerea on human colorectal cancer cells (COLO 205). Journal of Current Science and Technology. Vol. 9 No.1, pp. 41-47.

Interpretive Summary: Vernonia cinerea, commonly known as iron weed, is a perennial herbaceous plant mainly distributed in tropical areas. The stem, bark, roots, leaves and flowers have been used traditionally as a folk remedy for many diseases. The plant parts are known to be rich sources of sesquiterpene lactones, a diverse group of biologically active plant chemicals. In this study, we demonstrated the anticancer activity of four hirsutinolide compounds isolated from V. cinerea against human colorectal cells. The anticancer activity of hirsutinolides in this study was associated with a position 13 hydrophobic ester side chain that inhibited cell viability and DNA synthesis. These natural products derived from V. cinerea expands our cache of biologically active constituents from tropical plants.

Technical Abstract: In this study, we investigated the effect of hirsutinolide series isolated from Vernonia cinerea on the cytotoxic and anti-apoptotic activity on human colorectal cancer cell lines (COLO 205). The results showed that hirsutinolide series significantly inhibited cell viability of COLO 205 with IC50 less than 1.5 µM. Flow cytometric analysis indicated that hirsutinolide series (compounds 7, 10, 11, and 22) inhibited DNA synthesis as evidenced by a decline of DNA content in S, G2/M, and G0/G1 phases of the cell cycle. Furthermore, hirsutinolide series provided anticancer activity through inducing apoptosis on COLO 205.