Location: Bioproducts ResearchTitle: Quantification of the molecular species of acylglycerols containing hydroxy fatty acids in lesquerella oils using high-performance liquid chromatography and mass spectrometry
Submitted to: Book Chapter
Publication Type: Book / Chapter
Publication Acceptance Date: 4/25/2017
Publication Date: 1/16/2018
Citation: Lin, J.T. Chen, G.Q. 2018. Castor and Lesquerella oils: production, composition, and uses. In: Chen, G.Q., editor. Quantification if the Molecular Species of Acylglycerols Containing Hydroxy Fatty Acids in Lesquerella Oil Using High-performance Liquid Chromatography and Mass Spectrometry. New York, NY: Nova Science Publisher Inc. p. 19-34.
Interpretive Summary: We have developed a new method to quantitate the molecular species of acylglycerols containing hydroxy fatty acids in vegetable oil. Hydroxy fatty acids can be used in industry in the manufacturing of lubricant, plastic, cosmetics etc. The molecular species of acylglycerols were quantified by high-performance liquid chromatography (HPLC) with evaporative light scattering detector and electrospray ionization mass spectrometry of the lithium adducts of the molecular species of acylglycerols in the HPLC fractions of lesquerella oil.
Technical Abstract: Ten molecular species of diacylglycerols (DAG), 54 of triacylglycerols (TAG) and 13 of tetraacylglycerols (tetraAG, triacylglycerol estolides) containing hydroxy fatty acids (FA) as well as 20 of TAG containing three normal FA (non-hydroxylated) in lesquerella oil were quantified by a newly improved method using HPLC with evaporative light scattering detector (ELSD) and electrospray ionization mass spectrometry (ESI-MS) of their lithium adducts from the HPLC fractions of lesquerella oil. Hydroxy FA such as lesquerolic acid (OH(superscript 14)20:1 (superscript 11) in lesquerella oil can be used in industry. The area % of the individual HPLC peak (with ELSD) of lesquerella oil was used as the total content (%) of the molecular species of acylglycerols (AG) in the HPLC peak. The ratio of the MS1 ion signal intensities from the different molecular species of AG in a HPLC peak was used as the ratio of the contents of the molecular species of AG in this HPLC peak. The area % of the HPLC peak and the ratio of the contents of the molecular species of AG in this HPLC peak were used to estimate the contents (%) of individual molecular species of AG. The quantification of the ratio of regioisomers of the molecular species of TAG in lesquerella oil was also introduced here. These identification and quantification of the molecular species of AG could help to elucidate their biosynthesis.