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ARS Home » Midwest Area » Madison, Wisconsin » Vegetable Crops Research » Research » Publications at this Location » Publication #339756

Research Project: Potato Genetic Resource Management, Characterization, and Evaluation

Location: Vegetable Crops Research

Title: Botanical seed technology at the US Potato Genebank

Author
item Bamberg, John

Submitted to: American Journal of Potato Research
Publication Type: Proceedings
Publication Acceptance Date: 4/17/2017
Publication Date: 6/1/2018
Citation: Bamberg, J.B. 2018. Botanical seed technology at the US Potato Genebank. American Journal of Potato Research. 95:209.

Interpretive Summary:

Technical Abstract: Studies on botanical seed technology have potential payoffs for genebank in-house operations as well as promoting efficient use of the germplasm by cooperators. When we tested the effects of soil fertilization, mother plants with extra fertilizer produced more fruit and seeds, but those extra seeds did not have greater seed weight or germination after 25 years of storage. When we tested method of emasculation, removing only anthers reduced fruit weight and seed yield compared to non-emasculated buds, and removing sepals, petals, and anthers reduced fruit- and seedset even more. When we tested the effect of crowding on GA seed pretreatment and growth in the germinating pot, we found no differences in germination when 25, 50 or 100 seeds were treated and sown in the same space. When some diploid wild species are crossed as females with tuberosum diploid males, only a small percentage of seeds are plump in the berries, and even those do not develop normally into germinable seeds. We found that embryos of such seeds often develop enough to be “rescued” by excising and culturing them on sterile media. Some seedlots take a long time to start germination, then only a few seeds germinate at a time, reaching a low final percent germination. We found that germinating such seedlots in alternating temperature (22od/7on) can greatly improve each of these parameters.