|GILIO, T - Universidade Estadual De Maringá|
|VALENTINI, GISELI - Universidade Estadual De Maringá|
|CASTRO, SANDRA - Universidade Estadual De Maringá|
|ELIAS, HAROLDO - Centro De Pesquisas Rene' Rachou|
|GONCALVES-GIDIGAL, CELESTE - Universidade Estadual De Maringá|
|Pastor Corrales, Marcial - Talo|
Submitted to: Bean Improvement Cooperative Annual Report
Publication Type: Trade Journal
Publication Acceptance Date: 3/30/2017
Publication Date: 4/1/2017
Citation: Gilio, T., Hurtado-Gonzales, O.P., Valentini, G., Castro, S.A., Elias, H., Song, Q., Goncalves-Gidigal, C.M., Pastor Corrales, M.A. 2017. Fine mapping the borad spectrum anthracnose resistance gene in Amendoim Cavalo. Bean Improvement Cooperative Annual Report. 60:3-4.
Technical Abstract: Anthracnose, caused by the fungus Colletotrichum lindemuthianum, is a major disease of bean in the USA and Brazil. Genetic resistance is the most cost-effective strategy to manage this disease. The Andean common bean Amendoim Cavalo (AC) is resistant to many races of C. lindemuthianum present in many countries of the Americas including the USA and Brazil. Previous results have shown that the resistance in AC is conferred by a single and dominant gene that is different from the other 14 named and mapped anthracnose resistance genes. The objective of this study was to use fine mapping to locate the position of the AC locus in the common bean genome. This study was conducted using 110 F2 seedlings from the cross PI 207262 (S) x AC (R) that were inoculated with the race 3481 of C. lindemuthianum. DNA of the two parents and resistant and susceptible F2 bulks were screened with the BARCBEAN6K_3 BeadChip containing 5,398 SNPs. Subsequently SSR and KASP markers, specifically designed to trace the AC locus in a smaller DNA region, were used to screen 700 F2:3 plants, which were also phenotyped with race 3481. The results from these studies showed that the AC resistance locus was flanked by KASP markers ss102 and ss95 in a 65.22 Kbp region on the lower arm of chromosome Pv01 of common bean. These studies also demonstrated that the AC locus was different from Co-x, another Andean anthracnose locus that has been also mapped in the region containing the AC locus.