Skip to main content
ARS Home » Midwest Area » Madison, Wisconsin » Vegetable Crops Research » Research » Publications at this Location » Publication #339596
Title: Different haplotypes encode the same protein for independent sources of zucchini yellow mosaic virus resistance in cucumber

Author
item RAMIREZ-MADERA, AXEL - University Of Wisconsin
item Havey, Michael

Submitted to: HortScience
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 6/29/2017
Publication Date: 8/1/2017
Publication URL: http://handle.nal.usda.gov/10113/5852172
Citation: Ramirez-Madera, A.O., Havey, M.J. 2017. Different haplotypes encode the same protein for independent sources of zucchini yellow mosaic virus resistance in cucumber. HortScience. 52(8):1040-1042. https://doi.org/10.21273/HORTSCI11951-17.

Interpretive Summary: Cucumber production is negatively affected by zucchini yellow mosaic virus (ZYMV). Three sources of ZYMV resistance have been commercially deployed and all three resistances are conditioned by a single recessive gene. A vacuolar protein sorting-associated protein 4-like (VPS4-like) gene has been proposed as a candidate for ZYMV resistance in cucumber. We analyzed the genomic sequence across the VPS4-like gene for three independent sources of ZYMV resistance in cucumber (A192-18, Dina-1 and TMG-1) and identified three haplotypes across the coding region and considerable variation in the introns. Haplotypes in the coding regions of the VPS4-like gene of A192-18, Dina-1 and TMG-1 encode the same protein sequence, revealing the genetic uniformity for ZYMV resistance from diverse germplasm sources. These results will be of use for cucumber breeders and pathologists in the public and private sectors to recognize that only one major source of ZYMV resistance is currently deployed.

Technical Abstract: Cucumber (Cucumis sativus) production is negatively affected by zucchini yellow mosaic virus (ZYMV). Three sources of ZYMV resistance have been commercially deployed and all three resistances are conditioned by a single recessive gene. A vacuolar protein sorting-associated protein 4-like (VPS4-like) gene has been proposed as a candidate for ZYMV resistance in cucumber. We sequenced the genomic region across the VPS4-like gene for three independent sources of ZYMV resistance in cucumber (A192-18, Dina-1 and TMG-1) and identified three haplotypes across the coding region and considerable variation in the introns. Haplotypes in the coding regions of the VPS4-like gene of A192-18, Dina-1 and TMG-1 encode the same protein sequence, revealing the genetic uniformity for ZYMV resistance from diverse germplasm sources.