Page Banner

United States Department of Agriculture

Agricultural Research Service

Research Project: Genetic Enhancement of Allium, Cucumis, and Daucus Germplasm

Location: Vegetable Crops Research

Title: Different haplotypes encode the same protein for independent sources of ZYMV resistance in cucumber

Author
item Ramirez-madera, Axel
item Havey, Michael

Submitted to: HortScience
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 6/29/2017
Publication Date: N/A
Citation: N/A

Interpretive Summary: Cucumber production is negatively affected by zucchini yellow mosaic virus (ZYMV). Three sources of ZYMV resistance have been commercially deployed and all three resistances are conditioned by a single recessive gene. A vacuolar protein sorting-associated protein 4-like (VPS4-like) gene has been proposed as a candidate for ZYMV resistance in cucumber. We analyzed the genomic sequence across the VPS4-like gene for three independent sources of ZYMV resistance in cucumber (A192-18, Dina-1 and TMG-1) and identified three haplotypes across the coding region and considerable variation in the introns. Haplotypes in the coding regions of the VPS4-like gene of A192-18, Dina-1 and TMG-1 encode the same protein sequence, revealing the genetic uniformity for ZYMV resistance from diverse germplasm sources. These results will be of use for cucumber breeders and pathologists in the public and private sectors to recognize that only one major source of ZYMV resistance is currently deployed.

Technical Abstract: Cucumber (Cucumis sativus) production is negatively affected by zucchini yellow mosaic virus (ZYMV). Three sources of ZYMV resistance have been commercially deployed and all three resistances are conditioned by a single recessive gene. A vacuolar protein sorting-associated protein 4-like (VPS4-like) gene has been proposed as a candidate for ZYMV resistance in cucumber. We sequenced the genomic region across the VPS4-like gene for three independent sources of ZYMV resistance in cucumber (A192-18, Dina-1 and TMG-1) and identified three haplotypes across the coding region and considerable variation in the introns. Haplotypes in the coding regions of the VPS4-like gene of A192-18, Dina-1 and TMG-1 encode the same protein sequence, revealing the genetic uniformity for ZYMV resistance from diverse germplasm sources.

Last Modified: 09/25/2017
Footer Content Back to Top of Page