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ARS Home » Southeast Area » Charleston, South Carolina » Vegetable Research » Research » Publications at this Location » Publication #339593

Research Project: Characterization, Etiology, and Disease Management for Vegetable Crops

Location: Vegetable Research

Title: Understanding the mechanism of resistance breaking on tomato by Tomato mottle mosaic virus

Author
item SUI, XUELIAN - Fujian Agriculture And Forest University
item Shamimuzzaman, Md
item ZHENG, YI - Boyce Thompson Institute
item Simmons, Alvin
item FEI, ZHANGJUN - Boyce Thompson Institute
item WU, ZUJIAN - Fujian Agriculture And Forest University
item Ling, Kai-Shu

Submitted to: American Phytopathological Society Annual Meeting
Publication Type: Abstract Only
Publication Acceptance Date: 5/5/2017
Publication Date: 12/1/2017
Citation: Sui, X., Shamimuzzaman, M., Zheng, Y., Simmons, A.M., Fei, Z., Wu, Z., Ling, K. 2017. Understanding the mechanism of resistance breaking on tomato by Tomato mottle mosaic virus. American Phytopathological Society Annual Meeting. 107(12S):S5.121. https://doi.org/10.1094/PHYTO-107-12-S5.1.
DOI: https://doi.org/10.1094/PHYTO-107-12-S5.1

Interpretive Summary: N/A

Technical Abstract: Tomato mottle mosaic virus (ToMMV) has broadened it’s distribution around the world. In our previous work, we observed a partial resistance breaking by ToMMV on tomato. To understand the mechanism of this resistance breaking, we carried out comparative analysis through Sanger sequencing, genotyping by sequencing and RNA sequencing. We confirmed the presence of Tm-22 gene in both susceptible (S) and resistant (R) cv. ‘B’ plants and observed no sequence variations. Next, in Genotyping-by-sequencing of over 190 cv. ‘B’ plants showing resistance (175) or susceptibility (17), we also could not identify any single nucleotide polymorphisms (SNPs) in association with the resistance breaking. Then, through profiling of global gene expression using RNA-seq between the R and S cv. ‘B’ plants, only 42 differentially expressed genes (DEGs) were identified. Gene Ontology analysis revealed that DEGs were enriched for catalytic and binding functions, with major classes involved in pathogenesis, transcription factors and proteases. However, there was no clear correlation of any particular genetic element to resistance breaking. Finally, a temperature test was conducted to investigate whether the resistance breaking was caused by higher temperature. We observed an increasing number of ToMMV-inoculated cv. ‘B’ plants with resistance breaking as temperature increased from 25' to 30' or 35'. This resistance breaking by higher temperature might have a significant impact on food security due to global warming.